Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Permanent Cell Line Cell
SUBMITTER: Jeovanis Gil
LAB HEAD: Sergio Manuel Encarnación-Guevara
PROVIDER: PXD005903 | Pride | 2017-09-12
REPOSITORIES: Pride
Action | DRS | |||
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JV_140624_Qexactive_CF1.raw | Raw | |||
JV_140624_Qexactive_CF2.raw | Raw | |||
JV_140624_Qexactive_CF3.raw | Raw | |||
JV_140624_Qexactive_CG1.raw | Raw | |||
JV_140624_Qexactive_CG2.raw | Raw |
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The Journal of biological chemistry 20170911 44
Lysine acetylation is a widespread posttranslational modification affecting many biological pathways. Recent studies indicate that acetylated lysine residues mainly exhibit low acetylation occupancy, but challenges in sample preparation and analysis make it difficult to confidently assign these numbers, limiting understanding of their biological significance. Here, we tested three common sample preparation methods to determine their suitability for assessing acetylation stoichiometry in three hu ...[more]