Dataset Information

Large-scale HDMSE mapping of the normal sputum proteome

ABSTRACT: Severe asthma is a collection of disease entities with varying pathophysiological characteristics that result in symptoms of cough, wheeze and breathlessness, with frequent exacerbations. To address the problem of phenotypic difference and heterogeneity, the Unbiased Biomarkers for the Prediction of Respiratory Disease Outcomes (U-BIOPRED) project was set up as a public-private partnership within the framework of the Innovative Medicines Initiative (IMI), engaging academia, the pharmaceutical industry and patient groups. The goal of this investigation was to identify proteomic fingerprints in induced sputum that characterize patients with severe asthma and to determine whether subgroups of severe asthmatics can be identified. Furthermore, we were interested in elucidating the biological pathways that showed differences between subgroups. This dataset is a description of the normal sputum proteome.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Sputum

SUBMITTER: Paul Skipp

LAB HEAD: Paul J S Skipp

PROVIDER: PXD005949 | Pride | 2018-05-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	UBIOPRED_ IA_running batch10_040413_UASPB4_085_8930_001_IA_final_fragment.pride.mgf.gz	Mgf
	UBIOPRED_ IA_running batch10_040413_UASPB4_085_8930_001_IA_final_fragment.pride.mztab.gz	Mztab
	UBIOPRED_ IA_running batch10_040413_UASPB4_085_8930_002_IA_final_fragment.pride.mgf.gz	Mgf
	UBIOPRED_ IA_running batch10_040413_UASPB4_085_8930_002_IA_final_fragment.pride.mztab.gz	Mztab
	UBIOPRED_ IA_running batch11_050413_UASPB5_092_8877_001_IA_final_fragment.pride.mgf.gz	Mgf

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Publications

Large-Scale Label-Free Quantitative Mapping of the Sputum Proteome.

Burg Dominic D Schofield James P R JPR Brandsma Joost J Staykova Doroteya D Folisi Caterina C Bansal Aruna A Nicholas Ben B Xian Yang Y Rowe Anthony A Corfield Julie J Wilson Susan S Ward Jonathan J Lutter Rene R Fleming Louise L Shaw Dominick E DE Bakke Per S PS Caruso Massimo M Dahlen Sven-Erik SE Fowler Stephen J SJ Hashimoto Simone S Horváth Ildikó I Howarth Peter P Krug Norbert N Montuschi Paolo P Sanak Marek M Sandström Thomas T Singer Florian F Sun Kai K Pandis Ioannis I Auffray Charles C Sousa Ana R AR Adcock Ian M IM Chung Kian Fan KF Sterk Peter J PJ Djukanović Ratko R Skipp Paul J PJ The U-Biopred Study Group

Journal of proteome research 20180523 6

Analysis of induced sputum supernatant is a minimally invasive approach to study the epithelial lining fluid and, thereby, provide insight into normal lung biology and the pathobiology of lung diseases. We present here a novel proteomics approach to sputum analysis developed within the U-BIOPRED (unbiased biomarkers predictive of respiratory disease outcomes) international project. We present practical and analytical techniques to optimize the detection of robust biomarkers in proteomic studies. ...[more]

PMID: 29737851

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