Proteomics

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Hickory LC-MS/MS - Comparative Proteomic Analysis of the Graft Unions in Hickory (Carya cathayensis) Provides Insights into Response Mechanisms to Grafting Process


ABSTRACT: Hickory (Carya cathayensis) is a woody plant with high nutritional and economic value, and is widely cultivated in China. It is different from herbaceous plants and undergoes a long vegetative stage before reaching floral transition. Hickory can be cultivated on a large scale using grafting. To reveal the response mechanisms to grafting, we employed a proteomics-based approach to identify differentially expressed proteins during the hickory grafting process. In our study, 3723 proteins were identified and 2518 proteins were quantified. A total of 710 differentially quantified proteins (DQPs) were identified and these were involved in various molecular functional and biological processes. Among these DQPs, 341 were up-regulated and 369 were down-regulated at 7 days after grafting compared to the control. The protein interaction networks of the DQPs indicated that there were two enriched interaction clusters. These were „ribosome‟ and „starch and sucrose metabolism‟. Four auxin-related proteins were down-regulated, which was in agreement with the transcription levels of their encoding genes. The Kyoto Encyclopedia of Genes and Genomes analysis showed that the „Flavonoid biosynthesis‟ pathway was significantly up-regulated. Further experiments verified a significant increase in the total flavonoid contents in scions, which suggested that graft union formation may activate flavonoid biosynthesis to increase the contents of a series of downstream secondary metabolites. This comprehensive analysis provides fundamental information on the candidate proteins and the secondary metabolism pathways involved in the grafting process for hickory.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Carya Cathayensis

TISSUE(S): Whole Body

SUBMITTER: zhang zhang  

LAB HEAD: Bingsong Zheng

PROVIDER: PXD006025 | Pride | 2018-10-26

REPOSITORIES: Pride

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Publications

Comparative Proteomic Analysis of the Graft Unions in Hickory (<i>Carya cathayensis</i>) Provides Insights into Response Mechanisms to Grafting Process.

Xu Dongbin D   Yuan Huwei H   Tong Yafei Y   Zhao Liang L   Qiu Lingling L   Guo Wenbin W   Shen Chenjia C   Liu Hongjia H   Yan Daoliang D   Zheng Bingsong B  

Frontiers in plant science 20170427


Hickory (<i>Carya cathayensis</i>), a tree with high nutritional and economic value, is widely cultivated in China. Grafting greatly reduces the juvenile phase length and makes the large scale cultivation of hickory possible. To reveal the response mechanisms of this species to grafting, we employed a proteomics-based approach to identify differentially expressed proteins in the graft unions during the grafting process. Our study identified 3723 proteins, of which 2518 were quantified. A total o  ...[more]

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