Dataset Information

T. brucei MAPKLK1 knockdown - The MAP kinase MAPKLK1 is essential to Trypanosoma brucei proliferation and regulates proteins involved in mRNA metabolism

ABSTRACT: Pathogenic trypanosomatids have a large number of protein kinases and phosphatases in comparison to other organisms. Moreover, post-translational modifications have key role in the gene expression control in these cells, reinforcing the relevance of the phosphorylation process. Nevertheless little is known about protein phosphorylation in these protozoa. In front of this, the effects of the depletion of a MAP kinase-like kinase (MAPKLK1) were evaluated in Trypanosoma brucei. After silencing MAPKLK1 expression by RNAi, the cells were evaluated by SILAC MS-based proteomics. In total, 1,756 phosphorylation sites were identified, of which 384 were not previously described in T. brucei. The modulations observed on proteome and phosphoproteome are related to key cellular processes enriched to mRNA processing and stability control.

INSTRUMENT(S): LTQ Orbitrap XL ETD

ORGANISM(S): Trypanosoma Brucei

TISSUE(S): Cell Suspension Culture

SUBMITTER: Michel Batista

LAB HEAD: Fabricio Klerynton Marchini

PROVIDER: PXD006048 | Pride | 2017-10-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	TbMAPKLK1_Phospho_R1_F1_1_1.RAW	Raw
	TbMAPKLK1_Phospho_R1_F1_1_2.RAW	Raw
	TbMAPKLK1_Phospho_R1_F1_2_1.RAW	Raw
	TbMAPKLK1_Phospho_R1_F1_2_2.RAW	Raw
	TbMAPKLK1_Phospho_R1_F1_3_1.RAW	Raw

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The MAP kinase MAPKLK1 is essential to Trypanosoma brucei proliferation and regulates proteins involved in mRNA metabolism.

Batista Michel M Kugeratski Fernanda G FG de Paula Lima Carla V CV Probst Christian M CM Kessler Rafael L RL de Godoy Lyris M LM Krieger Marco A MA Marchini Fabricio K FK

Journal of proteomics 20161227

Protein phosphorylation and dephosphorylation events regulate many cellular processes. The identification of all phosphorylation sites and their association to a respective protein kinase or phosphatase is a challenging and crucial step to have a deeper understanding of the effects of signaling networks on cells. Pathogenic trypanosomatids have a large number of protein kinases and phosphatases in comparison to other organisms, which reinforces the relevance of the phosphorylation process in the ...[more]

PMID: 28039027

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