Proteomics

Dataset Information

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Proteomic analysis of Glycine max seeds during controlled deterioration treatment


ABSTRACT: Controlled deterioration treatment (CDT) negatively affects the seed quality and vigor during post-harvest storage. A label-free proteomic approach was utilized to understand the CDT responses in soybean seeds. Soybean seed are rich in seed-storage proteins (SSPs) constituting up to 70 to 80% of the total seed protein content. Due to the presence of these SSPs, it is very difficult to identify and/or characterize the low-abundance regulatory proteins. Availability of appropriate methods for extraction of low-abundance proteins (LAPs) are now providing a platform for the identification of novel proteins involved in the signal perception and transduction during environmental perturbations. To enrich LAPs, the extracted total seed proteins were subjected to protamine sulfate precipitation (PSP) method to deplete SSPs. Fractionated protein fractions thus prepared were analyzed for identification of differential proteins using Label-free quantitative proteomics approaches.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Glycine Max

TISSUE(S): Seed

SUBMITTER: Sun Tae Kim  

LAB HEAD: Sun Tae Kim

PROVIDER: PXD006064 | Pride | 2017-10-10

REPOSITORIES: Pride

Dataset's files

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Action DRS
RAW3day_1.raw Raw
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Publications

Gel-based and gel-free proteome data associated with controlled deterioration treatment of <i>Glycine max</i> seeds.

Min Cheol Woo CW   Lee Seo Hyun SH   Cheon Ye Eun YE   Han Won Young WY   Ko Jong Min JM   Kang Hang Won HW   Kim Yong Chul YC   Agrawal Ganesh Kumar GK   Rakwal Randeep R   Gupta Ravi R   Kim Sun Tae ST  

Data in brief 20171002


Data presented here are associated with the article: "In-depth proteomic analysis of soybean (<i>Glycine max</i>) seeds during controlled deterioration treatment (CDT) reveals a shift in seed metabolism" (Min et al., 2017) [1]. Seed deterioration is one of the major problems, affecting the seed quality, viability, and vigor in a negative manner. Here, we display the gel-based and gel-free proteomic data, associated with the CDT in soybean seeds. The present data was obtained from 2-DE, shotgun p  ...[more]

Publication: 1/2

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