Dataset Information

Ovarian Cancer Lipid raft proteomics and role of OPCML

ABSTRACT: ipid rafts are dynamic membrane micro-domains that orchestrate molecular interactions and are implicated in cancer development. To understand potential role of tumor suppressor OPCML on the altered dynamics of lipid raft residing proteins, we performed quantitative (SILAC) proteomics experiment. We recently found that OPCML negatively regulates a subset of receptor tyrosine kinases (RTKs) by altering their recycling and ubiquitin-mediated degradation via sequestration to lipid rafts; however, the molecular mechanism linking OPCML tumor suppressor expression to altered RTK trafficking remains unclear. Here we performed a quantitative lipid raft proteomics study to dissect raft-mediated mechanism of OPCML-regulated tumor suppression. We also examined raft-associated mechanism of OPCML P95R, a common mutation resulting in substitution of proline to arginine at position 95, mediated regulation of adhesion potential of tumors.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Anup Shah

LAB HEAD: Michelle M. Hill

PROVIDER: PXD006078 | Pride | 2017-03-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20110526_OPCMLSILAC3_1_1.mzXML	Mzxml
	20110526_OPCMLSILAC3_1_1.pep.xml	Pepxml
	20110526_OPCMLSILAC3_1_10.mzXML	Mzxml
	20110526_OPCMLSILAC3_1_10.pep.xml	Pepxml
	20110526_OPCMLSILAC3_1_11.mzXML	Mzxml

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Publications

Integrative Analysis of Subcellular Quantitative Proteomics Studies Reveals Functional Cytoskeleton Membrane-Lipid Raft Interactions in Cancer.

Shah Anup D AD Inder Kerry L KL Shah Alok K AK Cristino Alexandre S AS McKie Arthur B AB Gabra Hani H Davis Melissa J MJ Hill Michelle M MM

Journal of proteome research 20160902 10

Lipid rafts are dynamic membrane microdomains that orchestrate molecular interactions and are implicated in cancer development. To understand the functions of lipid rafts in cancer, we performed an integrated analysis of quantitative lipid raft proteomics data sets modeling progression in breast cancer, melanoma, and renal cell carcinoma. This analysis revealed that cancer development is associated with increased membrane raft-cytoskeleton interactions, with ∼40% of elevated lipid raft proteins ...[more]

PMID: 27384440

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