Proteomics

Dataset Information

0

Composite PRC1/dBRD4/MOZ/MORF protein complexes in Drosophila


ABSTRACT: In this project, we provide strong proteomic evidence for PcG interaction with classic co-activators, BRD4 and MOZ/MORF, captured on chromatin during embryogenesis in Drosophila, leading to a model in which developmental regulatory elements are universally ‘poised’ early in development via occupancy of composite protein complexes of PcG silencing proteins and classical co-activators. These bivalent protein interactions may resolve into full activation or repression, depending on the cell type-specific expression, binding, and function of transcription factors.

OTHER RELATED OMICS DATASETS IN: PRJNA408128GSE104059

INSTRUMENT(S): LTQ Orbitrap Velos, LTQ

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo, Cell Culture

SUBMITTER: Barry Zee  

LAB HEAD: Mitzi Kuroda

PROVIDER: PXD006079 | Pride | 2017-11-02

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
272098_Barry_CG_INPUT.mzid Mzid
272098_Barry_CG_INPUT.pep.xml Pepxml
272099_Barry_CG_Strep.mzid Mzid
272099_Barry_CG_Strep.pep.xml Pepxml
272231_Barry_Sfmbt_Input.mzid Mzid
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Publications

Bivalent complexes of PRC1 with orthologs of BRD4 and MOZ/MORF target developmental genes in <i>Drosophila</i>.

Kang Hyuckjoon H   Jung Youngsook L YL   McElroy Kyle A KA   Zee Barry M BM   Wallace Heather A HA   Woolnough Jessica L JL   Park Peter J PJ   Kuroda Mitzi I MI  

Genes & development 20171025 19


Regulatory decisions in <i>Drosophila</i> require Polycomb group (PcG) proteins to maintain the silent state and Trithorax group (TrxG) proteins to oppose silencing. Since PcG and TrxG are ubiquitous and lack apparent sequence specificity, a long-standing model is that targeting occurs via protein interactions; for instance, between repressors and PcG proteins. Instead, we found that Pc-repressive complex 1 (PRC1) purifies with coactivators Fs(1)h [female sterile (1) homeotic] and Enok/Br140 dur  ...[more]

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