Dataset Information

Composite PRC1/dBRD4/MOZ/MORF protein complexes in Drosophila

ABSTRACT: In this project, we provide strong proteomic evidence for PcG interaction with classic co-activators, BRD4 and MOZ/MORF, captured on chromatin during embryogenesis in Drosophila, leading to a model in which developmental regulatory elements are universally ‘poised’ early in development via occupancy of composite protein complexes of PcG silencing proteins and classical co-activators. These bivalent protein interactions may resolve into full activation or repression, depending on the cell type-specific expression, binding, and function of transcription factors.

OTHER RELATED OMICS DATASETS IN: GSE104059PRJNA408128

INSTRUMENT(S): LTQ Orbitrap Velos, LTQ

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo, Cell Culture

SUBMITTER: Barry Zee

LAB HEAD: Mitzi Kuroda

PROVIDER: PXD006079 | Pride | 2017-11-02

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	272098_Barry_CG_INPUT.mzid	Mzid
	272098_Barry_CG_INPUT.pep.xml	Pepxml
	272099_Barry_CG_Strep.mzid	Mzid
	272099_Barry_CG_Strep.pep.xml	Pepxml
	272231_Barry_Sfmbt_Input.mzid	Mzid

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Publications

Bivalent complexes of PRC1 with orthologs of BRD4 and MOZ/MORF target developmental genes in <i>Drosophila</i>.

Kang Hyuckjoon H Jung Youngsook L YL McElroy Kyle A KA Zee Barry M BM Wallace Heather A HA Woolnough Jessica L JL Park Peter J PJ Kuroda Mitzi I MI

Genes & development 20171025 19

Regulatory decisions in <i>Drosophila</i> require Polycomb group (PcG) proteins to maintain the silent state and Trithorax group (TrxG) proteins to oppose silencing. Since PcG and TrxG are ubiquitous and lack apparent sequence specificity, a long-standing model is that targeting occurs via protein interactions; for instance, between repressors and PcG proteins. Instead, we found that Pc-repressive complex 1 (PRC1) purifies with coactivators Fs(1)h [female sterile (1) homeotic] and Enok/Br140 dur ...[more]

PMID: 29070704

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