Proteomics

Dataset Information

0

Label free comparative proteome analysis of Arabidopsis lipid droplets from 6 week old leaves


ABSTRACT: Lipid droplets from 6 week old Arabidopsis leaves were purified on sucrose gradient. The abundance of the proteins in the lipid droplet fraction was established by label free and compared to soluble, membrane and plastoglobule fractions, and total leaf extract in order to identify the proteins specifically enriched in lipid droplets. Three independent biological replicates, from three independent cultures were performed. For each biological replicates, three preparations of lipid droplets (and other fractions) within each of the three independent cultures were obtained.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Stephane Claverol  

LAB HEAD: Claire Brehelin

PROVIDER: PXD006113 | Pride | 2017-06-19

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
cb140521_02b.RAW Raw
cb140521_02b.msf Msf
cb140521_03b.RAW Raw
cb140521_03b.msf Msf
cb140521_03c.RAW Raw
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Publications

Proteomic Analysis of Lipid Droplets from Arabidopsis Aging Leaves Brings New Insight into Their Biogenesis and Functions.

Brocard Lysiane L   Immel Françoise F   Coulon Denis D   Esnay Nicolas N   Tuphile Karine K   Pascal Stéphanie S   Claverol Stéphane S   Fouillen Laëtitia L   Bessoule Jean-Jacques JJ   Bréhélin Claire C  

Frontiers in plant science 20170529


Lipid droplets (LDs) are cell compartments specialized for oil storage. Although their role and biogenesis are relatively well documented in seeds, little is known about their composition, structure and function in senescing leaves where they also accumulate. Here, we used a label free quantitative mass spectrometry approach to define the LD proteome of aging Arabidopsis leaves. We found that its composition is highly different from that of seed/cotyledon and identified 28 proteins including 9 e  ...[more]

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