Dataset Information

Discovery of Mouse Spleen Signaling Responses to Anthrax using Label-Free Quantitative Phosphoproteomics via Mass Spectrometry

ABSTRACT: From article abstract: Inhalational anthrax is caused by spores of the bacterium Bacillus anthracis (B. anthracis), and is an extremely dangerous disease that can kill unvaccinated victims within 2 weeks. Modern antibiotic-based therapy can increase the survival rate to ~50%, but only if administered presymptomatically (within 24-48 h of exposure). To discover host signaling responses to presymptomatic anthrax, label-free quantitative phosphoproteomics via liquid chromatography coupled to mass spectrometry was used to compare spleens from uninfected and spore-challenged mice over a 72 h time-course. Late-stage (48-72 h post-challenge) Sterne strain (lethal) infections resulted in global alterations to the spleen phosphoproteome. In contrast, delta-Sterne strain (asymptomatic; missing the anthrax toxin) infections resulted in 188 (5.8%) significantly altered (q<0.05) phosphopeptides. Twenty-six highly tentative phosphorylation responses to early-stage (24 h post-challenge) anthrax were discovered (q<0.5), and ten of these originated from eight proteins that have known roles in the host immune response. These tentative early-anthrax host response signaling events within mouse spleens may translate into presymptomatic diagnostic biomarkers of human anthrax detectable within circulating immune cells, and could aid in the identification of pathogenic mechanisms and therapeutic targets. [Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.000927, 1–11, 2011. The files in this dataset are exactly the same files that were originally uploaded to Proteome Commons Tranche as described in the article.]

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Bacillus Anthracis Str. Sterne Mus Musculus (mouse)

TISSUE(S): Spleen

DISEASE(S): Anthrax Disease

SUBMITTER: Nathan Manes

LAB HEAD: Serguei Popov

PROVIDER: PXD006148 | Pride | 2017-03-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	030809_01_TiO2TS7_400ngBetaCasein_Trypsin_8pmolAngIIP_TiO2_1pmolAngI_LTQ-Orb.RAW	Raw
	030809_02_TiO2TS6_400ngBetaCasein_Trypsin_8pmolAngIIP_TiO2_1pmolAngI_LTQ-Orb.RAW	Raw
	030809_05_TiO2TS5_400ngBetaCasein_Trypsin_8pmolAngIIP_TiO2_1pmolAngI_LTQ-Orb.RAW	Raw
	070109_02_C1-03_SpleenD1S1_500ngBetaCasein_Trypsin_8pmolAngIIP_TiO2_1pmolAngI_LTQ-Orb.RAW	Raw
	070109_03_C1-04_SpleenD1D1_500ngBetaCasein_Trypsin_8pmolAngIIP_TiO2_1pmolAngI_LTQ-Orb.RAW	Raw

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Publications

Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry.

Manes Nathan P NP Dong Li L Zhou Weidong W Du Xiuxia X Reghu Nikitha N Kool Arjan C AC Choi Dahan D Bailey Charles L CL Petricoin Emanuel F EF Liotta Lance A LA Popov Serguei G SG

Molecular & cellular proteomics : MCP 20101228 3

Inhalational anthrax is caused by spores of the bacterium Bacillus anthracis (B. anthracis), and is an extremely dangerous disease that can kill unvaccinated victims within 2 weeks. Modern antibiotic-based therapy can increase the survival rate to ∼50%, but only if administered presymptomatically (within 24-48 h of exposure). To discover host signaling responses to presymptomatic anthrax, label-free quantitative phosphoproteomics via liquid chromatography coupled to mass spectrometry was used to ...[more]

PMID: 21189417

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