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Definition of a high-confidence mitochondrial proteome at quantitative scale_pure versus crude mitochondria

ABSTRACT: Mitochondria perform central functions in cellular bioenergetics, metabolism and signaling and their malfunction has been linked to numerous diseases. The available studies cover only part of the mitochondrial proteome and a separation of core mitochondrial proteins from associated fractions has not been achieved. We developed an integrative, quantitative MS-based experimental approach to define the high confidence proteome of yeast mitochondria and to identify new mitochondrial proteins. The analysis includes protein abundance under fermentable and non-fermentable growth, submitochondrial localization, single-protein analysis and subcellular classification of mitochondria-associated fractions. We identified novel mitochondrial interactors of respiratory chain supercomplexes, ATP synthase, AAA proteases, the mitochondrial contact site and cristae organizing system (MICOS) and coenzyme Q biosynthesis cluster as well as new mitochondrial proteins with dual cellular localization. The integrative proteome provides a high confidence source for the characterization of physiological and pathophysiological functions of mitochondria and their integration into the cellular environment.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Friedel Drepper

LAB HEAD: Prof. Dr. Bettina Warscheid

PROVIDER: PXD006151 | Pride | 2017-06-29

REPOSITORIES: Pride

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Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale.

Morgenstern Marcel M Stiller Sebastian B SB Lübbert Philipp P Peikert Christian D CD Dannenmaier Stefan S Drepper Friedel F Weill Uri U Höß Philipp P Feuerstein Reinhild R Gebert Michael M Bohnert Maria M van der Laan Martin M Schuldiner Maya M Schütze Conny C Oeljeklaus Silke S Pfanner Nikolaus N Wiedemann Nils N Warscheid Bettina B

Cell reports 20170601 13

Mitochondria perform central functions in cellular bioenergetics, metabolism, and signaling, and their dysfunction has been linked to numerous diseases. The available studies cover only part of the mitochondrial proteome, and a separation of core mitochondrial proteins from associated fractions has not been achieved. We developed an integrative experimental approach to define the proteome of yeast mitochondria. We classified > 3,300 proteins of mitochondria and mitochondria-associated fractions ...[more]

PMID: 28658629

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