Proteomics

Dataset Information

0

Mice liver tissue LC-MSMS EPAC

ABSTRACT: We employed mouse models with targeted deletion of the genes encoding Epac1 (Rapgef3) and Epac2 (Rapgef4) in combination with 2/3 partial hepatectomy to investigate the functional roles of Epac1 and Epac2 in liver. Proteomic analysis of liver tissue before and 36h after hepatectomy revealed changes in the proteome in Epac depleted mice, most significantly of proteins involved in lipid metabolism. The current study is the first to identify functional roles for Epac1 and Epac2 in livers of an animal model. The results support the notion that Epac proteins are not required for normal liver development and function, but that these factors become important in the regulation of cellular responses in stressful situations.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Elise Aasebo  

LAB HEAD: Marit Bakke

PROVIDER: PXD006165 | Pride | 2019-09-12

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20151208_Sample10_dKO_47_regenerert.raw Raw
20151208_Sample11_dKO_55_L1.raw Raw
20151208_Sample12_dKO_55_regenerert.raw Raw
20151208_Sample13_1WT.raw Raw
20151208_Sample14_2WT_Reg.raw Raw
Items per page:
1 - 5 of 18
altmetric image

Publications

Mice depleted for Exchange Proteins Directly Activated by cAMP (Epac) exhibit irregular liver regeneration in response to partial hepatectomy.

Sivertsen Åsrud Kathrine K   Pedersen Line L   Aesoy Reidun R   Muwonge Haruna H   Aasebø Elise E   Nitschke Pettersen Ina Katrine IK   Herfindal Lars L   Dobie Ross R   Jenkins Stephen S   Berge Rolf Kristian RK   Henderson Neil Cowan NC   Selheim Frode F   Døskeland Stein Ove SO   Bakke Marit M  

Scientific reports 20190924 1

The exchange proteins directly activated by cAMP 1 and 2 (Epac1 and Epac2) are expressed in a cell specific manner in the liver, but their biological functions in this tissue are poorly understood. The current study was undertaken to begin to determine the potential roles of Epac1 and Epac2 in liver physiology and disease. Male C57BL/6J mice in which expression of Epac1 and/or Epac2 are deleted, were subjected to partial hepatectomy and the regenerating liver was analyzed with regard to lipid ac  ...[more]

Similar Datasets

Transcriptomics Wild type (WT), partial hepatectomy, and lncHand2 KO chip
2017-04-29 | GSE98357 | GEO
Transcriptomics Transcription profiling of mouse liver regeneration after partial hepatectomy
2008-06-13 | E-GEOD-4528 | biostudies-arrayexpress
Transcriptomics Scaling of gene expression with liver cell size in vivo
2014-03-06 | E-MTAB-1297 | biostudies-arrayexpress
Proteomics Deletion of mTOR in liver epithelial cells enhances hepatic metastasis of colon cancer
2023-01-05 | PXD019998 | Pride
Transcriptomics RNA-seq transcriptonal profiling in partial hepatectomy liver tissues in Arid1a WT and liver specific KO mice.
2016-03-30 | E-GEOD-76926 | biostudies-arrayexpress
Proteomics Proteomic characterization of hepatic expression signatures affected in ZHX2 liver conditional knockout mice
2023-10-17 | PXD027897 | Pride
Transcriptomics RNA-seq transcriptonal profiling in partial hepatectomy liver tissues in Arid1a WT and liver specific KO mice.
2016-03-30 | GSE76926 | GEO
Transcriptomics Consequences of beta-PDGFR deletion on hepatic stellate cells during hepatic regeneration
2013-11-08 | E-GEOD-52234 | biostudies-arrayexpress
Genomics microRNA expression profiling of the normal and regenerating liver of Chiloscyllium plagiosum
2016-08-30 | GSE72339 | GEO
Transcriptomics Effect of conditional deletion of S6 ribosomal protein following partial hepatectomy
2009-05-01 | E-GEOD-13864 | biostudies-arrayexpress