Proteomics

Dataset Information

0

Phosphoproteome and proteome of T. cruzi metacyclogenesis


ABSTRACT: Trypanosoma cruzi metacyclogenesis is a natural process that occurs inside triatomine insect vector and corresponds to the differentiation of non-infective epimastigotes into infective metacyclic trypomastigotes. Here, centered on LC-MS/MS approach, a large scale phospho (proteome) study was carried out aiming to quantitatively understand the metacyclogenesis. Cells were colected along metacyclogenesis totalizing six points, stress 2 hours, adhered 12, 24 and 48 hours, and metacyclic trypomastigote from two biological replicates. After extraction, total protein were digested, fractioned and analyzed by LC-MS/MS. The samples for phosphoproteomics were processed for phosphopeptide enrichment prior to the analysis.

INSTRUMENT(S): LTQ Orbitrap XL ETD

ORGANISM(S): Trypanosoma Cruzi Dm28c

SUBMITTER: juliana carolina amorim  

LAB HEAD: Fabricio Klerynton Marchini

PROVIDER: PXD006171 | Pride | 2017-08-31

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Non_phospho.zip Other
Phospho.zip Other
Tc_Phospho_Ad12hR1_1_1.RAW Raw
Tc_Phospho_Ad12hR1_1_2.RAW Raw
Tc_Phospho_Ad12hR1_2_1.RAW Raw
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Publications

Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis.

Amorim Juliana C JC   Batista Michel M   da Cunha Elizabeth S ES   Lucena Aline C R ACR   Lima Carla V de Paula CVP   Sousa Karla K   Krieger Marco A MA   Marchini Fabricio K FK  

Scientific reports 20170829 1


Trypanosoma cruzi metacyclogenesis is a natural process that occurs inside the triatomine vector and corresponds to the differentiation of non-infective epimastigotes into infective metacyclic trypomastigotes. The biochemical alterations necessary for the differentiation process have been widely studied with a focus on adhesion and nutritional stress. Here, using a mass spectrometry approach, a large-scale phospho(proteome) study was performed with the aim of understanding the metacyclogenesis p  ...[more]

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