Dataset Information

Impact of the short chain fatty acid, sodium butyrate, on gut resident microbiota, gut mucus proteome and fish susceptibility to bacterial and parasitic infections in vivo

ABSTRACT: Previous works in the framework of EU ARRAINA Project evidenced a pro-inflammatory condition in gilthead sea bream (Sparus aurata) fed extremely low fish meal/fish oil diets, and this effect was mostly reversed by butyrate supplementation. The hypothesis of work is that these nutritionally-mediated changes can be extensive to intestinal mucus proteome and gut microbiota, which in turn could modify disease outcome.s If so, the prevalence and progression of the disease might be also modified by diet composition and feed additives. Gilthead sea bream fingerlings were fed with control and experimental diets formulated by BioMar until two year-old. FM was added at 25% in the control diet (D1) and at 5% in the other three diets (D2-D4). Added oil was either FO (D1 control diet) or a blend of vegetable oils, replacing the 58% (D2) and the 84% (D3-D4 diets) of FO. A commercial sodium butyrate preparation (NOREL, BP70) was added to the D4 diet at 0.4%. At month 20, 6 fish per each dietary treatment were sampled for iTRAQ profiling and fingerprinting of intestinal mucus proteome. Mucus collected from anterior and posterior intestine segments was trypsin digested, labelled with iTRAQ reagents, isoelectrofocused and resolved by LC-MS/MS. More than 1000 proteins were unequivocally annotated and principal component analysis clearly separated anterior and posterior segments. The diet effect with changes in the abundance of approximately 120 proteins was restricted to anterior section with a reversion of the pattern of the extreme diet (D3 fish) with dietary butyrate supplementation. Butyrate supplementation also reversed the decrease of microbiotay diversity associated with D3 feeding, and led to a improvement the disease outcomes in fish challenged with Photobacterium damselae and the intestinal parasite Enteromyxum leei.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Sparus Aurata

TISSUE(S): Skin Mucus, Epithelial Cell

SUBMITTER: Luz Valero

LAB HEAD: Jaume Pérez-Sánchez

PROVIDER: PXD006183 | Pride | 2018-10-24

REPOSITORIES: Pride

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Publications

Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets.

Piazzon María Carla MC Calduch-Giner Josep Alvar JA Fouz Belén B Estensoro Itziar I Simó-Mirabet Paula P Puyalto Mónica M Karalazos Vasileios V Palenzuela Oswaldo O Sitjà-Bobadilla Ariadna A Pérez-Sánchez Jaume J

Microbiome 20171228 1

<h4>Background</h4>The constant increase of aquaculture production and wealthy seafood consumption has forced the industry to explore alternative and more sustainable raw aquafeed materials, and plant ingredients have been used to replace marine feedstuffs in many farmed fish. The objective of the present study was to assess whether plant-based diets can induce changes in the intestinal mucus proteome, gut autochthonous microbiota and disease susceptibility of fish, and whether these changes cou ...[more]

PMID: 29282153

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