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Pig GIT meatproteome - Dietary changes in nutritional studies shape the structural and functional composition of the pigs' fecal microbiome-from days to weeks

ABSTRACT: Background: The possible impact of changes in diet composition for the intestinal microbiome is mostly studied after some days of adaptation to the diet of interest. The question arises if few days are enough to reflect the microbial response to the diet by changing the community composition and function. The present study investigated the fecal microbiome of pigs in a time span of four weeks after a dietary change to get an insight of the needed adaptation period. Four different diets were used differing in either protein source (field peas meal vs. soybean meal) or the concentration of calcium and phosphorus (CaP). Results: Twelve pigs were sampled at seven time points within four weeks after the dietary change. Fecal samples were used to sequence the 16S rDNA amplicons, to analyse the microbial proteins via LC-MS/MS and to determine the SCFA production. The analysis of OTU abundances and quantification values of proteins showed a significant separation of three periods of time (p=0.001). Samples from the first day are used to define the ‘Zero phase’, samples of weeks one and two are combined as ‘metabolic phase’ and an ‘equilibrium phase’ was defined based on samples from week three and four. Only in this last phase, a separation according to the supplementation of CaP was significantly detectable (p=0.001). No changes were found based on the corn-soybean meal or corn-field peas administration. The analysis of possible factors causing this significant separation showed only an overall change of bacterial members and functional properties. The metaproteomic approach yields a total of about 9700 proteins, which were used to deduce possible metabolic functions of the bacterialcommunity.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bacteria Sus Scrofa Domesticus (domestic Pig)

SUBMITTER: Jana Seifert

LAB HEAD: Jana Seifert

PROVIDER: PXD006224 | Pride | 2017-10-26

REPOSITORIES: Pride

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Dietary changes in nutritional studies shape the structural and functional composition of the pigs' fecal microbiome-from days to weeks.

Tilocca Bruno B Burbach Katharina K Heyer Charlotte M E CME Hoelzle Ludwig E LE Mosenthin Rainer R Stefanski Volker V Camarinha-Silva Amélia A Seifert Jana J

Microbiome 20171027 1

<h4>Background</h4>The possible impact of changes in diet composition on the intestinal microbiome is mostly studied after some days of adaptation to the diet of interest. The question arises if a few days are enough to reflect the microbial response to the diet by changing the community composition and function. The present study investigated the fecal microbiome of pigs during a time span of 4 weeks after a dietary change to obtain insights regarding the time required for adaptation. Four diff ...[more]

PMID: 29078812

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			Action	DRS
	0.1.01.raw	Raw
	0.1.03.raw	Raw
	0.1.04.raw	Raw
	0.1.05.raw	Raw
	0.1.06.raw	Raw