Dataset Information

Protein profile of mouse ovarian follicles grown in vitro

ABSTRACT: This project presents a protein catalog of the in vitro growing mouse ovarian follicle and it provides insights about proteomics throughout in vitro follicle development.

INSTRUMENT(S): HCTultra

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Ovarian Follicle, Ovary

SUBMITTER: Cédric Pionneau

LAB HEAD: Solenne Chardonnet

PROVIDER: PXD006227 | Pride | 2017-10-31

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	1112A_IEF01_J0-ET_LCT01.mgf	Mgf
	1112A_IEF01_J0-ET_LCT01.xml	Xml
	1112A_IEF01_J0-ET_LCT03.mgf	Mgf
	1112A_IEF01_J0-ET_LCT03.mzML	Mzml
	1112A_IEF01_J0-ET_LCT03.xml	Xml

Items per page:

1 - 5 of 550

Publications

Protein profile of mouse ovarian follicles grown in vitro.

Anastácio Amandine A Rodriguez-Wallberg Kenny A KA Chardonnet Solenne S Pionneau Cédric C Fédérici Christian C Almeida Santos Teresa T Poirot Catherine C

Molecular human reproduction 20171201 12

<h4>Study question</h4>Could the follicle proteome be mapped by identifying specific proteins that are common or differ between three developmental stages from the secondary follicle (SF) to the antrum-like stage?<h4>Summary answer</h4>From a total of 1401 proteins identified in the follicles, 609 were common to the three developmental stages investigated and 444 were found uniquely at one of the stages.<h4>What is known already</h4>The importance of the follicle as a functional structure has be ...[more]

PMID: 29069483

Similar Datasets

Project description:The relevance of immune-endocrine interactions to the regulation of ovarian function in teleosts is virtually unexplored. As part of the innate immune response during infection, a number of cytokines such as tumor necrosis factor Î± (TNFÎ±) and other immune factors, are produced and act on the reproductive system. However, TNFÎ± is also an important physiological player in the ovulatory process in mammals. In the present study, we have examined for the first time the effects of TNFÎ± in vitro in preovulatory ovarian follicles of a teleost fish, the brown trout (Salmo trutta). In control and recombinant trout TNFÎ± (rtTNFÎ±)-treated granulosa cells, we examined the percentage of apoptosis by flow cytometry analysis and cell viability by propidium iodide (PI) staining. Furthermore, we determined the in vitro effects of rtTNFÎ± on follicle contraction and testosterone production in preovulatory trout ovarian follicles. In addition, we analyzed the gene expression profiles of control and rtTNFÎ±-treated ovarian tissue by microarray and real-time PCR (qPCR) analyses.Treatment with rtTNFÎ± induces ovarian cell apoptosis, decreases granulosa cell viability and stimulates the expression of genes known to be involved in the normal ovulatory process in trout. In addition, rtTNFÎ± causes a significant increase in follicle contraction and testosterone production. Also, using a salmonid-specific microarray platform (SFA2.0 immunochip) we observed that rtTNFÎ± induces the expression of genes known to be involved in inflammation, proteolysis and tissue remodeling. In view of these results, we propose that TNFÎ± could have an important role in the biomechanics of follicle weakening, ovarian rupture and oocyte expulsion during ovulation in trout, primarily through its stimulation of follicular cell apoptosis and the expression of genes involved in follicle wall proteolysis and contraction. Reproductively mature female brown trout (Salmo trutta) from a cultured stock at the Piscifactoria de BagÃ (Generalitat de Catalunya, BagÃ , Spain) were kept under natural conditions of temperature and photoperiod. Fish at the preovulatory stage (according to the position of the germinal vesicle (GV) in oocytes that were cleared using a solution previously described), were anesthesized in 3-aminobenzoic acid ethyl ester (0.1 g/l; Sigma, Alcobendas, Spain) dissolved in fresh water, and the fish were sacrificed by concussion prior to the collection of the ovaries. The dissected ovaries were immediately used for the various in vitro assays. After dissection, brown trout preovulatory ovaries were placed in HankÂ´s balanced salt solution (HBSS) and individual ovarian follicles were manually separated with forceps from each ovary on ice, as previously described. To collect ovarian tissue for RNA extraction, preovulatory follicles from each of a total of three females were incubated (400 follicles/50 ml) in HBSS-BSA in the absence or presence of rtTNFÎ± (100 ng/ ml, dissolved directly in HBSS-BSA), at 15ÂºC for 24 h with gentle shaking (100 rpm). At the end of the incubation follicles (previously de-yolked by gentle pressure) were removed, flash frozen in liquid nitrogen and stored at -80ÂºC until assayed.