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Navel Leaf Early Detection - Longitudinal transcriptomic, proteomic, and metabolomic analyses of Citrus sinensis (L

ABSTRACT: Navel orange trees were grafted with budwood infected with the citrus greening bacterium, 'Candidatus Liberibacter asiaticus', or control budwood, and leaf samples were collected every two weeks post graft for LC/MS analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Citrus Sinensis (sweet Orange) (citrus Aurantium Var. Sinensis)

TISSUE(S): Leaf

SUBMITTER: Kevin Howe

LAB HEAD: Michelle Cilia

PROVIDER: PXD006316 | Pride | 2020-01-08

REPOSITORIES: Pride

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			Action	DRS
	20170101_NavelLeaf_Rep1_10_NH1_26.mgf	Mgf
	20170101_NavelLeaf_Rep1_10_NH1_26.mzid.gz	Mzid
	20170101_NavelLeaf_Rep1_10_NH1_26.pride.mgf.gz	Mgf
	20170101_NavelLeaf_Rep1_10_NH1_26.pride.mztab.gz	Mztab
	20170101_NavelLeaf_Rep1_10_NH1_26.raw	Raw

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Publications

Longitudinal Transcriptomic, Proteomic, and Metabolomic Analyses of Citrus sinensis (L.) Osbeck Graft-Inoculated with "Candidatus Liberibacter asiaticus".

Chin Elizabeth L EL Ramsey John S JS Mishchuk Darya O DO Saha Surya S Foster Elizabeth E Chavez Juan D JD Howe Kevin K Zhong Xuefei X Polek MaryLou M Godfrey Kris E KE Mueller Lukas A LA Bruce James E JE Heck Michelle M Slupsky Carolyn M CM

Journal of proteome research 20200115 2

"Candidatus Liberibacter asiaticus" (CLas) is the bacterium associated with the citrus disease Huanglongbing (HLB). Current CLas detection methods are unreliable during presymptomatic infection, and understanding CLas pathogenicity to help develop new detection techniques is challenging because CLas has yet to be isolated in pure culture. To understand how CLas affects citrus metabolism and whether infected plants produce systemic signals that can be use ...[more]

PMID: 31885275

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Citrus Proteomics: Lemon CLas graft inoculation Week 2

Project description:Lisbon lemon trees were grafted with budwood infected with the citrus greening bacterium, 'Candidatus Liberibacter asiaticus', or control budwood, and leaf samples were collected every two weeks post graft for LC/MS analysis. This project has shotgun proteomics data for leaf samples from 5 control and 5 CLas grafted trees at the two week post graft timepoint.

2021-09-07 | PXD006011 | Pride

Citrus Proteomics: Lemon CLas graft inoculation Week 14

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Expression data of sweet orange in response to Candidatus Liberibacter asiaticus infection at advanced stage

Project description:Citrus Huanglongbing (HLB, or greening) is one of the most severe diseases of citrus. Plant disease symptom development is considered to be the consequence of a number of molecular, cellular and physiological changes, and may also be associated with host defense responses. Understanding citrus host response to HLB may contribute to the development of new strategies to control this destructive disease. We performed microarray analysis to identify the differentially expressed genes in sweet orange in response to HLB infection using the Affymetrix GeneChipM-BM-. citrus genome array. Two-year-old seedlings of M-bM-^@M-^XMadam VinousM-bM-^@M-^Y sweet orange (Citrus sinensis L. Osbeck) were inoculated by grafting with bud sticks from HLB-diseased, PCR positive sweet orange plants. For mock-inoculated controls, the same types of plants were grafted with bud sticks from HLB-free, PCR negative sweet orange. At 7 months after inoculation, mature leaves were sampled from 3 individual HLB-diseased plants, and healthy leaves from 3 mock-inoculated plants as control. Total RNA was extracted from leaf samples and hybridized on Affymetrix microarrays.

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Response of Sweet Orange (Citrus sinensis) to Candidatus Liberibacter asiaticus Infection

Project description:Citrus greening or huanglongbing (HLB) is a devastating disease of citrus. HLB is associated with the phloem-limited fastidious prokaryotic alpha-proteobacterium Candidatus Liberibacter spp. In this report, we used sweet orange (Citrus sinensis) leaf tissue infected with 'Ca. Liberibacter asiaticus' and compared this with healthy controls. Investigation of the host response was examined with citrus microarray hybridization based on 30,171 sets expressed sequence tag sequences from several citrus species and hybrids. The microarray analysis indicated that HLB infection significantly affected expression of 624 genes whose encoded proteins were categorized according to function. The categories included genes associated with sugar metabolism, plant defense, phytohormone, and cell wall metabolism, as well as 14 other gene categories. Young, healthy Valencia sweet orange (C. sinensis) plants were graft inoculated with budwood from Ca. L. asiaticus-infected citrus plants. Prior to the innocualtion, the plants were confirmed to be Ca. L. asiaticus-free in ordinary and quantitative PCR tests. The presence of the bacteria in the inoculated plants was confirmed in both conventional and quantitative PCR with specific primers to Ca. L. asiaticus. The stem and root samples used for RNA extraction and hybridization on Affymetrix microarrays were obtained from three symptomatic and three healthy control trees of similar size, approximately 1 year after inoculation.

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Transcriptome dynamics during fruit development of sweet orange (Citrus sinensis)

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Transcriptome dynamics during fruit development of sweet orange (Citrus sinensis)

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