Proteomics

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Determination of the specificity profiling of native and synthetic cardosin B using proteomic identification of protease cleavage sites (PICS)

ABSTRACT: We performed an analysis of the substrate specificity preferences of native and synthetic cardosin B using the Proteomic Identification of protease Cleavage Sites methodology and some differences regarding preference and stringency of the subsite binding pockets were observed. Results clearly suggest that synthetic cardosin B is more stringent than native cardosin B explaining the observed suitability of the synthetic protein as biotechnological tool in cheese industry.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Blood Cell, Monocyte, Blood

SUBMITTER: Rui Cruz  

LAB HEAD: Isaura Simões

PROVIDER: PXD006329 | Pride | 2018-05-04

REPOSITORIES: Pride

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Dataset's files

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Action DRS
20140802_BWO_GluC_10uLof17uL_009.mzXML Mzxml
20140802_BWO_GluC_10uLof17uL_009.wiff Wiff
20140802_BWO_GluC_10uLof17uL_009.wiff.scan Wiff
20140802_BWO_GluC_1uLof17uL_SET18-20140802_BWO_GluC_1uLof17uL_008.mzXML Mzxml
20140802_BWO_GluC_1uLof17uL_SET18.wiff Wiff
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Publications

Functional and structural characterization of synthetic cardosin B-derived rennet.

Almeida Carla Malaquias CM   Manso José A JA   Figueiredo Ana C AC   Antunes Liliana L   Cruz Rui R   Manadas Bruno B   Bur Daniel D   Pereira Pedro José Barbosa PJB   Faro Carlos C   Simões Isaura I  

Applied microbiology and biotechnology 20170802 18

The potential of using a synthetic cardosin-based rennet in cheese manufacturing was recently demonstrated with the development and optimization of production of a recombinant form of cardosin B in Kluyveromyces lactis. With the goal of providing a more detailed characterization of this rennet, we herein evaluate the impact of the plant-specific insert (PSI) on cardosin B secretion in this yeast, and provide a thorough analysis of the specificity requirements as well as the biochemical and struc  ...[more]

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