Proteomics

Dataset Information

0

Arabidopsis thaliana DOG1 pull-downs from seed tissues


ABSTRACT: Functionally complemented dog1-1 mutant with a YFP-DOG1 fusion protein under the control of its native promoter were used as starting material for in vivo pull-down of YFP: DOG1 and its interacting proteins from seed tissues in native conditions. DOG1 interacting proteins were identified by mass spectrometry. Pull-downs were performed in biological triplicates using dry or 24 h water imbibed seeds, directly after harvest (dormant condition) and after 7 months of dry storage (non-dormant condition).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Seed

SUBMITTER: Katharina Kramer  

LAB HEAD: Prof. Iris Finkemeier

PROVIDER: PXD006347 | Pride | 2017-07-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
140707_WS_GuNe_TQS01.raw Raw
140707_WS_GuNe_TQS02.raw Raw
140707_WS_GuNe_TQS03b.raw Raw
140707_WS_GuNe_TQS04b.raw Raw
140707_WS_GuNe_TQS05b.raw Raw
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Publications

DELAY OF GERMINATION1 requires PP2C phosphatases of the ABA signalling pathway to control seed dormancy.

Née Guillaume G   Kramer Katharina K   Nakabayashi Kazumi K   Yuan Bingjian B   Xiang Yong Y   Miatton Emma E   Finkemeier Iris I   Soppe Wim J J WJJ  

Nature communications 20170713 1


The time of seed germination is a major decision point in the life of plants determining future growth and development. This timing is controlled by seed dormancy, which prevents germination under favourable conditions. The plant hormone abscisic acid (ABA) and the protein DELAY OF GERMINATION 1 (DOG1) are essential regulators of dormancy. The function of ABA in dormancy is rather well understood, but the role of DOG1 is still unknown. Here, we describe four phosphatases that interact with DOG1  ...[more]

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