Dataset Information

Fast hyperbaric decompression after heliox saturation altered the brain proteome in rats

ABSTRACT: In order to better understand physiological mechanisms and neurological symptoms involved in the development of decompression sickness we determined effects in rats on the brain proteome of fast decompression (1 bar/20 s) compared to controls (1 bar/10 min) after heliox saturation diving. The orbitrap LC-MS/MS data files presented here resulted in 1062 proteins quantified using label-free proteomics. Based on the 128 significantly regulated proteins in the orbitrap dataset and 56 in an iontrap dataset (967 quantified proteins), the networks “synaptic vesicle fusion and recycling in nerve terminals” and “translation initiation” were significantly enriched in a system biological database analysis (Metacore). Ribosomal proteins (RLA2, RS10) and the proteins hippocalcin-like protein 4 and proteasome subunit beta type-7 were significantly upregulated in both datasets. The heat shock protein 105 kDa, Rho-associated protein kinase 2 and Dynamin-1 were significantly downregulated in both datasets.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Alvhild Alette Bjoerkum

LAB HEAD: Alvhild Alette Bjørkum

PROVIDER: PXD006349 | Pride | 2017-10-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Bjorkum_DivingRats_Progenesispeptides.xlsx	Xlsx
	Bjorkum_DivingRats_Progenesissearchandquantificationresults.xlsx	Xlsx
	FD10_AH_15B_12.raw	Raw
	FD1_AH_6B_D1.raw	Raw
	FD2_AH_7B_D2.raw	Raw

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Publications

Fast hyperbaric decompression after heliox saturation altered the brain proteome in rats.

Bjørkum Alvhild Alette AA Oveland Eystein E Stuhr Linda L Havnes Marianne Bjordal MB Berven Frode F Grønning Marit M Hope Arvid A

PloS one 20171004 10

Better understanding of the physiological mechanisms and neurological symptoms involved in the development of decompression sickness could contribute to improvements of diving procedures. The main objective of the present study was to determine effects on the brain proteome of fast decompression (1 bar/20 s) compared to controls (1 bar/10 min) after heliox saturation diving, using rats in a model system. The protein S100B, considered a biomarker for brain injury, was not significantly different ...[more]

PMID: 28977037

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