Proteomics

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An optimised method for CSF extracellular vesicle extraction and comparative proteomic analysis

ABSTRACT: Extracellular vesicles (EVs) are implicated in the pathogenesis of neurodegenerative diseases and offer a source of biomarkers for diagnostic, stratification and therapeutic monitoring purposes. A major limitation in the development of specific biomarkers based on cerebrospinal fluid (CSF) EVs is the difficulty of extracting sufficient quantities of vesicles for discovery techniques such as proteomics. This study describes a novel application of a size-based extraction technique for CSF EVs using ultrafiltration liquid chromatography (UFLC), and comparison with the gold-standard ultracentrifugation (UC) method.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebrospinal Fluid

DISEASE(S): Multiple Sclerosis,Normal Pressure Hydrocephalus,Encephalopathy,Narcolepsy,Intracranial Hypertension,Peripheral Neuropathy

SUBMITTER: Marie-Laëtitia Thézénas  

LAB HEAD: Pr. Benedikt Kessler

PROVIDER: PXD006353 | Pride | 2018-11-30

REPOSITORIES: pride

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Dataset's files

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F273173_MLT_FL0323_MSQ797_EGray_120916_exosomes.mzid.gz Mzid
F273173_MLT_FL0323_MSQ797_EGray_120916_exosomes.pride.mztab.gz Mztab
F276216_MLT_FL0323_MSQ797_EGray_120916_SN_progA.mzid.gz Mzid
F276216_MLT_FL0323_MSQ797_EGray_120916_SN_progA.pride.mztab.gz Mztab
F276217_MLT_FL0323_MSQ797_EGray_120916_SN_progB.mzid.gz Mzid
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Publications

Cerebrospinal fluid (CSF) extracellular vesicles (EVs) show promise as a source of neurological disease biomarkers, although their precise origin is poorly understood. Current extraction techniques produce disappointing yield and purity. This study describes the application of ultrafiltration LC (UFLC) to CSF-EVs, compared with ultracentrifugation (UC), and explores CSF-EV origin. EVs are extracted from human CSF by UC and UFLC and characterized using nanoparticle tracking analysis, electron mic  ...[more]

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