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In depth proteome profiling of Cumin by LTQ-Orbitrap Velos mass spectrometer

ABSTRACT: A shotgun proteomic approach was used to obtain comprehensive dataset of cumin proteins. For this purpose we applied a one dimensional electrophoresis coupled with high resolution mass spectrometry. The first step in our proteome analysis strategy, we evaluate different extraction methods, based on differential solubility of protein. Among those methods, Readyprep protein extraction kit provide high purity of protein extract, gave higher protein yield and typically good resolution of protein bands on one dimensional gel electrophoresis. Further nanoLC-MS/MS analysis by state-of-the-art linear ion trap (LTQ)-Orbitrap Velose mass spectrometer resulted in identification of very high number proteins. Bioinformatics analysis provide functional distribution of identified protein. To our knowledge, this is the first study aimed for in depth study of cumin proteome.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Uzma Rana

LAB HEAD: Uzma Zaman

PROVIDER: PXD006425 | Pride | 2022-02-28

REPOSITORIES: Pride

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			Action	DRS
	Cumin_Velose_Kit_1.raw	Raw
	Cumin_Velose_Kit_10.raw	Raw
	Cumin_Velose_Kit_11.raw	Raw
	Cumin_Velose_Kit_12.raw	Raw
	Cumin_Velose_Kit_13.raw	Raw

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