Dataset Information

Exhaled breath condensate biomarkers for the early diagnosis of lung cancer using proteomics

ABSTRACT: The present study explored whether the proteomic analysis of exhaled breath condensate (EBC) may provide potential biomarkers for their use in non-invasive screening strategies for the early detection of lung cancer (LC). EBC was collected from 192 individuals (49 control volunteers, 49 risk factor-smoking volunteers, 46 chronic obstructive pulmonary disease patients and 48 LC patients). Using LC-MS/MS a total of 348 different proteins with a different pattern among the four groups were identified in EBC samples. Despite the great variability observed among individuals, significantly more proteins were identified in the EBC from LC patients compared to other groups. Furthermore, the average number of proteins identified per sample was significantly higher in LC patients and Receiver Operating Characteristic curve analysis showed an area under the curve of 0.8, indicating diagnostic value. Proteins frequently detected in EBC, such as dermcidin and hornerin, along with others much less frequently detected, such as hemoglobin and histone isoforms, were identified. Cytokeratins (KRTs) were the most abundant proteins in EBC samples and levels of KRT6A, KRT6B and KRT6C isoforms were significantly higher in samples from LC patients. Moreover, the amount of most KRTs in EBC samples from LC patients showed a significant positive correlation with tumor size. Finally, we used a random forest algorithm to generate a robust model using EBC protein data for the diagnosis of patients with LC. Thus, this study demonstrates that the proteomic analysis of EBC samples is an appropriated approach to develop biomarkers for the diagnosis of lung cancer.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung

SUBMITTER: Luz Valero

LAB HEAD: Antonio Rodríguez-Ariza

PROVIDER: PXD006436 | Pride | 2019-08-16

REPOSITORIES: Pride

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			Action	DRS
	ARA_140530.100.wiff	Wiff
	ARA_140530.100.wiff.scan	Wiff
	ARA_140530.100_MGFPeaklist.mgf	Mgf
	ARA_140530.100_MGFPeaklist.pride.mgf.gz	Mgf
	ARA_140530.101.wiff	Wiff

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Exhaled breath condensate biomarkers for the early diagnosis of lung cancer using proteomics.

López-Sánchez Laura M LM Jurado-Gámez Bernabé B Feu-Collado Nuria N Valverde Araceli A Cañas Amanda A Fernández-Rueda José L JL Aranda Enrique E Rodríguez-Ariza Antonio A

American journal of physiology. Lung cellular and molecular physiology 20170615 4

We explored whether the proteomic analysis of exhaled breath condensate (EBC) may provide biomarkers for noninvasive screening for the early detection of lung cancer (LC). EBC was collected from 192 individuals [49 control (C), 49 risk factor-smoking (S), 46 chronic obstructive pulmonary disease (COPD) and 48 LC]. With the use of liquid chromatography and tandem mass spectrometry, 348 different proteins with a different pattern among the four groups were identified in EBC samples. Significantly ...[more]

PMID: 28619761

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