Dataset Information

Immune repertoire after immunization as seen by next generation sequencing and proteomics

ABSTRACT: We study the repertoire of immunoglobulin sequences after immunization in a cohort of rats. Animals were immunized with dinitrophenol modified KLH or with recombinant human HuD. In the immune repertoire, and also in the matching proteomics data, we observe that the animals produce a repertoire that has many shared motifs for those immunized with the same antigen. Cluster analysis allows the samples to be segregated according to the immunogen used.

OTHER RELATED OMICS DATASETS IN: PRJNA386462

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Blood Serum

SUBMITTER: Martijn van Duijn

LAB HEAD: Theo Luider

PROVIDER: PXD006484 | Pride | 2017-10-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	091204OTc2_MvD-AffDNP1_063.RAW	Raw
	091204OTc2_MvD-AffDNP1_063.mgf	Mgf
	091204OTc2_MvD-AffDNP1_063.pride.mgf.gz	Mgf
	091204OTc2_MvD-AffDNP2_078.RAW	Raw
	091204OTc2_MvD-AffDNP2_078.mgf	Mgf

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Publications

Immune responses are characterized by specific shared immunoglobulin peptides that can be detected by proteomic techniques.

VanDuijn Martijn M MM Dekker Lennard J M LJ Zeneyedpour L L Smitt Peter A E Sillevis PA Luider Theo M TM

The Journal of biological chemistry 20100708 38

In the adaptive immune response, immunoglobulins develop that bind specifically to the antigens to which the organism was exposed. Immunoglobulins may bind to known or unknown antigens in a variety of diseases and have been used in the past to identify novel antigens for use as a biomarker. We propose that the immunoglobulins themselves could also be used as biomarkers in antibody-mediated disease. In this proteomic study, rats were immunized with one of two purified antigens, and immunoglobulin ...[more]

PMID: 20615873

Publication: 1/2

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