Proteomics

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Proteomics analysis of streptomyces sp. MBT76 demonstrates the production of qinichelins, new mixed catecholate-hydroxamate siderophores

ABSTRACT: Actinomycete genomes contain a plethora of orphan gene clusters encoding unknown secondary metabolites, and representing a huge unexploited pool of chemical diversity. The explosive increase in genome sequencing and the massive advance of bioinformatic tools have revolutionized the rationale for natural product discovery from actinomycetes. In this context, we applied a genome mining approach to discover a group of unique catecholate-hydroxamate siderophores termed as qinichelins from Streptomyces sp. MBT76. Quantitative proteomics statistically correlated a gene cluster of interest (qch) to its unknown chemotype (qinichelin), after which structural elucidation of isolated qinichelin was assisted by bioinformatics analysis and verified by MS2 and NMR experiments. Strikingly, intertwined functional crosstalk among four separately located gene clusters was implicated in the biosynthesis of qinichelins.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Streptomyces Sp. Mbt76

SUBMITTER: Jacob Gubbens  

LAB HEAD: Prof.dr. G.P. van Wezel

PROVIDER: PXD006577 | Pride | 2018-10-24

REPOSITORIES: Pride

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JG_QL109_1_03.RAW Raw
JG_QL109_1_04.RAW Raw
JG_QL109_1_05.RAW Raw
JG_QL109_1_06.RAW Raw
JG_QL109_1_07.RAW Raw
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Publications

Intertwined Precursor Supply during Biosynthesis of the Catecholate-Hydroxamate Siderophores Qinichelins in Streptomyces sp. MBT76.

Gubbens Jacob J   Wu Changsheng C   Zhu Hua H   Filippov Dmitri V DV   Florea Bogdan I BI   Rigali Sébastien S   Overkleeft Herman S HS   van Wezel Gilles P GP  

ACS chemical biology 20171002 11

The explosive increase in genome sequencing and the advances in bioinformatic tools have revolutionized the rationale for natural product discovery from actinomycetes. In particular, this has revealed that actinomycete genomes contain numerous orphan gene clusters that have the potential to specify many yet unknown bioactive specialized metabolites, representing a huge unexploited pool of chemical diversity. Here, we describe the discovery of a novel group of catecholate-hydroxamate siderophores  ...[more]

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