Dataset Information

Comparative degradomics of porcine and human wound exudates unravels biomarker candidates for assessment of wound healing progression in trauma patients

ABSTRACT: Impaired cutaneous wound healing is a major complication in elderly people and patients suffering from diabetes with raising rates in industrialized countries. Heterogeneity of clinical manifestations hampers effective molecular diagnostics and decisions for appropriate therapeutic regimens. Using a customized positional quantitative proteomics workflow, we have established a time-resolved proteome and N-terminome resource from wound exudates in a clinical pig wound model that we exploited as robust template to interpret a heterogeneous dataset from patients undergoing the same wound treatment. With Zyxin, IQGAP1 and HtrA1, this analysis and validation by targeted proteomics identified differential abundances and proteolytic processing of proteins of epidermal and dermal origin as prospective biomarker candidates for assessment of critical turning points in wound progression. Thus, we demonstrate the power of a fine-tuned animal wound model to bridge the translational gap as prerequisite for future extended clinical studies with large cohorts of individuals affected by healing impairments.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (human) Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Wound Fluid

DISEASE(S): Wounds And Injuries

SUBMITTER: Ulrich auf dem Keller

LAB HEAD: Ulrich auf dem Keller

PROVIDER: PXD006674 | Pride | 2017-09-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20130124_03_pig161_PO2.mgf	Mgf
	20130124_03_pig161_PO2.raw	Raw
	20130124_04_pig161_PO1.mgf	Mgf
	20130124_04_pig161_PO1.raw	Raw
	20130124_05_pig161_PO3.mgf	Mgf

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Publications

Comparative Degradomics of Porcine and Human Wound Exudates Unravels Biomarker Candidates for Assessment of Wound Healing Progression in Trauma Patients.

Sabino Fabio F Egli Fabian E FE Savickas Simonas S Holstein Jörg J Kaspar Daniela D Rollmann Mika M Kizhakkedathu Jayachandran N JN Pohlemann Tim T Smola Hans H Auf dem Keller Ulrich U

The Journal of investigative dermatology 20170909 2

Impaired cutaneous wound healing is a major complication in elderly people and patients suffering from diabetes, the rate of which is rising in industrialized countries. Heterogeneity of clinical manifestations hampers effective molecular diagnostics and decisions for appropriate therapeutic regimens. Using a customized positional quantitative proteomics workflow, we have established a time-resolved proteome and N-terminome resource from wound exudates in a clinically relevant pig wound model th ...[more]

PMID: 28899681

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