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Tyrosine phosphatase interaction network

ABSTRACT: Protein tyrosine phosphatases are crucial for various biological processes including cell metabolism, gene transcription, translation, cell-cycle regulation, protein stability, signal transduction, and apoptosis. To understand the functional network of each protein phosphatase in the cell, we performed a systematic proteomic analysis of 81 human protein phosphatases using tandem affinity purification coupled with mass spectrometry (TAP-MS).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Subbareddy Maddika

LAB HEAD: Dr. Maddika Subba Reddy

PROVIDER: PXD006698 | Pride | 2018-10-24

REPOSITORIES: Pride

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Publications

A Human Tyrosine Phosphatase Interactome Mapped by Proteomic Profiling.

Kumar Parveen P Munnangi Prathyusha P Chowdary Kvs Rammohan KR Shah Varun J VJ Shinde Swapnil R SR Kolli Nanci R NR Halehalli Rachita R RR Nagarajaram Hampapathalu A HA Maddika Subbareddy S

Journal of proteome research 20170718 8

Tyrosine phosphatases play a critical role in many cellular processes and pathogenesis, yet comprehensive analysis of their functional interacting proteins in the cell is limited. By utilizing a proteomic approach, here we present an interaction network of 81 human tyrosine phosphatases built on 1884 high-confidence interactions of which 85% are unreported. Our analysis has linked several phosphatases with new cellular processes and unveiled protein interactions genetically linked to various hum ...[more]

PMID: 28675297

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