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Direct Interactions of RNA Polymerase with Ribosomes and Ribosomal Subunits in E. Coli

ABSTRACT: The interactions between RNA polymerase and ribosomes are critical for the coordination of transcription with translation. We report that RNA polymerase directly binds ribosomes and isolated large and small ribosomal subunits.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Escherichia Coli

SUBMITTER: Preston Williams

LAB HEAD: Gregor M. Blaha

PROVIDER: PXD006717 | Pride | 2017-08-28

REPOSITORIES: Pride

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			Action	DRS
	30S-RNAP_BottomBand_TrisAcetate_T1.raw	Raw
	30S-RNAP_BottomBand_TrisAcetate_T2.raw	Raw
	30S-RNAP_BottomBand_TrisAcetate_T3.raw	Raw
	30S-RNAP_TopBand_TrisAcetate_T1.raw	Raw
	30S-RNAP_TopBand_TrisAcetate_T2.raw	Raw

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Publications

Transcription-translation coupling: direct interactions of RNA polymerase with ribosomes and ribosomal subunits.

Fan Haitian H Conn Adam B AB Williams Preston B PB Diggs Stephen S Hahm Joseph J Gamper Howard B HB Hou Ya-Ming YM O'Leary Seán E SE Wang Yinsheng Y Blaha Gregor M GM

Nucleic acids research 20171101 19

In prokaryotes, RNA polymerase and ribosomes can bind concurrently to the same RNA transcript, leading to the functional coupling of transcription and translation. The interactions between RNA polymerase and ribosomes are crucial for the coordination of transcription with translation. Here, we report that RNA polymerase directly binds ribosomes and isolated large and small ribosomal subunits. RNA polymerase and ribosomes form a one-to-one complex with a micromolar dissociation constant. The form ...[more]

PMID: 28977553

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