Dataset Information

PDGF-hSyn Synaptosomes (vGlut FASS) nanoLC-MS/MS

ABSTRACT: This project studies the differential abundance of proteins at excitatory synapses of PDGF-hSyn mice (Masliah et al., 2000, Science), which are a model for Parkinson’s disease/dementia with Lewy bodies. The mice overexpress human alpha synuclein under the human PDGF promoter. Synapses were purified from animals expressing fluorescently tagged vGlut1 (vGlut1-Venus) using fluorescence activated synapse sorting (FASS; cf. Biesemann et al., 2014, EMBO J).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Mario Dorostkar

LAB HEAD: Mario Dorostkar

PROVIDER: PXD006812 | Pride | 2019-09-02

REPOSITORIES: Pride

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Publications

Early defects in translation elongation factor 1α levels at excitatory synapses in α-synucleinopathy.

Blumenstock Sonja S Angelo Maria Florencia MF Peters Finn F Dorostkar Mario M MM Ruf Viktoria C VC Luckner Manja M Crux Sophie S Slapakova Lenka L Arzberger Thomas T Claverol Stéphane S Herzog Etienne E Herms Jochen J

Acta neuropathologica 20190826 6

Cognitive decline and dementia in neurodegenerative diseases are associated with synapse dysfunction and loss, which may precede neuron loss by several years. While misfolded and aggregated α-synuclein is recognized in the disease progression of synucleinopathies, the nature of glutamatergic synapse dysfunction and loss remains incompletely understood. Using fluorescence-activated synaptosome sorting (FASS), we enriched excitatory glutamatergic synaptosomes from mice overexpressing human alpha-s ...[more]

PMID: 31451907

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