Proteomics

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First insights into the pleiotropic role of vrf (yedF), a newly characterized gene of Salmonella Typhimurium


ABSTRACT: Salmonella Typhimurium is an enteric food-borne pathogen responsible for causing salmonellosis associated with acute diarrhea. The bacterial invasion initially requires flagellar motility to reach the intestinal lumen and to cross the mucus layer of the intestinal epithelia and subsequent adhesion to the host cell, mainly mediated by fimbriae .The key genes involved in the pathogenic process are encoded within highly conserved regions of the bacterial genome called Salmonella Pathogenicity Islands (SPIs). The key regulator HilA, which is positively regulated by HilC and HilD, is located in SPI-1;however, other virulence-related regulators, such as RtsA, are encoded outside this island . During the invasion, proteins encoded by the prgHIJK, spaMNOPQRS, and invABCEFGH SPI-1 operons constitute a Type-3 Secretion System. Effector proteins,such as SipA and SipC (encoded in SPI-1) and SigD/SopB (encoded in SPI-5), translocate to the host cytosol through this system causing intracellular changes and inducing the immune response . The survival and replication of intracellular Salmonella inside Salmonella-containing vacuoles (SCVs) is mainly mediated by the genes located in SPI-2. The immune response is activated through the recognition of pathogen-associated molecular patterns (PAMPs) by specific receptors expressed on the surface and/or inside different cell types. The most important PAMPs are flagellin (monomers comprising the flagella filaments), particularly the FliC subunit, which is highly expressed on the bacterial surface, and lipopolysaccharide (LPS).Oxygen availability is limited in the inflamed gut whereas other compounds, such as hydrogen sulfide (H2S) and H2 are abundantly produced by the colonic bacteria. In addition, nutrients are also limited inside the SCV, while reactive oxygen as wellas nitrogen species may be found . In anaerobic conditions, Salmonella is able to survive by using alternative energy sources, such as nitrate or fumarate, through the use of specific enzymes: nitrate and nitrite reductases, fumarate reductase , DMSO reductase and respiratory hydrogenases.In a previous study, we compared transcriptomes of a clinical isolate of S. Typhimurium (strain 50-wt) and its derivative-multidrug-resistant mutant (strain 50-64) using microarrays . In addition to showing antimicrobial resistance, strain was also less invasive. In the present study, we focused on the genes of unknown function that showed impaired expression. We further selected those genes with a putative role in the acquired resistance phenotype or in the observed repressed virulence observed. Here, 4 putative membrane proteins and the yedF of unknown function were investigated to evaluate their involvement inthese two phenotypes.

OTHER RELATED OMICS DATASETS IN: PRJNA393682

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Salmonella Enterica Subsp. Enterica Serovar Typhimurium Str. Sl1344

SUBMITTER: Ramon Díaz  

LAB HEAD: De Oliveira, Eliandre

PROVIDER: PXD006825 | Pride | 2018-10-24

REPOSITORIES: Pride

Dataset's files

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Action DRS
reg1346_iTRAQ_10raw.msf Msf
reg1346_iTRAQ_FTwash_150626174717.raw Raw
reg1346_iTRAQ_f1_150626184359.raw Raw
reg1346_iTRAQ_f2.raw Raw
reg1346_iTRAQ_f3.raw Raw
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First insights into the pleiotropic role of vrf (yedF), a newly characterized gene of Salmonella Typhimurium.

Ballesté-Delpierre Clara C   Fernandez-Orth Dietmar D   Ferrer-Navarro Mario M   Díaz-Peña Ramón R   Odena-Caballol Antonia A   Oliveira Eliandre E   Fàbrega Anna A   Vila Jordi J  

Scientific reports 20171110 1


Salmonella possesses virulence determinants that allow replication under extreme conditions and invasion of host cells, causing disease. Here, we examined four putative genes predicted to encode membrane proteins (ydiY, ybdJ, STM1441 and ynaJ) and a putative transcriptional factor (yedF). These genes were identified in a previous study of a S. Typhimurium clinical isolate and its multidrug-resistant counterpart. For STM1441 and yedF a reduced ability to interact with HeLa cells was observed in t  ...[more]

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