Proteomics

Dataset Information

0

SWATH-MS analysis of transgenic tobacco overexpressing 3-hydroxy-3-methylglutaryl-CoA synthase


ABSTRACT: 3-Hydroxy-3-methylglutaryl-CoA synthase (HMGS) is the second enzyme in the mevalonate pathway. While the recombinant Brassica juncea HMGS1 (BjHMGS1) mutant S359A displayed 10-fold higher enzyme activity than wild-type (wt) BjHMGS1, transgenic tobacco overexpressing S359A (OE-S359A) exhibited greater sterol content, growth rate and seed yield than OE-wtBjHMGS1. To explore the mechanism of HMGS and its mutant (S359A) in promotion of plant growth, SWATH-MS quantitative proteomics analysis was performed.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Nicotiana Tabacum (common Tobacco)

TISSUE(S): Seedling

SUBMITTER: Pan Liao  

LAB HEAD: Mee-Len Chye

PROVIDER: PXD006869 | Pride | 2021-01-04

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20150722WT-602_NtabBX-20150722.xlsx Xlsx
20150724402-602_NtabBX.xlsx Xlsx
20150724WT-402_NtabBXZ-scoretest.xlsx Xlsx
20170703WT-402libthrough.group Other
Lib402-1.wiff Wiff
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Publications

Overexpression and Inhibition of 3-Hydroxy-3-Methylglutaryl-CoA Synthase Affect Central Metabolic Pathways in Tobacco.

Liao Pan P   Lung Shiu-Cheung SC   Chan Wai Lung WL   Hu Menglong M   Kong Geoffrey Kwai-Wai GK   Bach Thomas J TJ   Hao Quan Q   Lo Clive C   Chye Mee-Len ML  

Plant & cell physiology 20210301 1


Little has been established on the relationship between the mevalonate (MVA) pathway and other metabolic pathways except for the sterol and glucosinolate biosynthesis pathways. In the MVA pathway, 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) catalyzes the condensation of acetoacetyl-CoA and acetyl-CoA to form 3-hydroxy-3-methylglutaryl-coenzyme A. Our previous studies had shown that, while the recombinant Brassica juncea HMGS1 (BjHMGS1) mutant S359A displayed 10-fold higher enzyme activity tha  ...[more]

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