Proteomics

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Bioactive natural product peptide discovery in fungal secretoms using PepSAVI-MS

ABSTRACT: Fungal secondary metabolites represent a rich and largely untapped source for bioactive molecules, including peptides with substantial structural diversity and pharmacological potential. As methods proceed to take a deep dive into fungal genomes, complimentary methods to identify bioactive components are required to keep pace with the expanding fungal repertoire. We developed PepSAVI-MS to expedite the search for natural product bioactive peptides and herein demonstrate proof-of-principle applicability of the pipeline for the discovery of bioactive peptides from fungal secretomes via identification of the antifungal killer toxin KP4 from Ustilago maydis P4. This work opens the door to investigating microbial secretomes with a new lens, and could have broad applications across human health, agriculture, and food safety.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Ustilago Maydis Virus P4

TISSUE(S): Cell Culture

SUBMITTER: Christine Kirkpatrick  

LAB HEAD: Leslie M. Hicks

PROVIDER: PXD006931 | Pride | 2018-02-19

REPOSITORIES: Pride

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20170703_CLK_BAP_UM_SCX2_3_elutionshift_10.wiff Wiff
20170703_CLK_BAP_UM_SCX2_3_elutionshift_10.wiff.scan Wiff
20170703_CLK_BAP_UM_SCX2_3_elutionshift_11.wiff Wiff
20170703_CLK_BAP_UM_SCX2_3_elutionshift_11.wiff.scan Wiff
20170703_CLK_BAP_UM_SCX2_3_elutionshift_12.wiff Wiff
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Publications

Fungal Secretome Analysis via PepSAVI-MS: Identification of the Bioactive Peptide KP4 from Ustilago maydis.

Kirkpatrick Christine L CL   Parsley Nicole C NC   Bartges Tessa E TE   Cooke Madeline E ME   Evans Wilaysha S WS   Heil Lilian R LR   Smith Thomas J TJ   Hicks Leslie M LM  

Journal of the American Society for Mass Spectrometry 20180205 5

Fungal secondary metabolites represent a rich and largely untapped source for bioactive molecules, including peptides with substantial structural diversity and pharmacological potential. As methods proceed to take a deep dive into fungal genomes, complimentary methods to identify bioactive components are required to keep pace with the expanding fungal repertoire. We developed PepSAVI-MS to expedite the search for natural product bioactive peptides and herein demonstrate proof-of-principle applic  ...[more]

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