Proteomics

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Performance evaluation of the Q Exactive HF-X for shotgun proteomics

ABSTRACT: Performance evaluation of the Q Exactive HF-X for shotgun proteomics

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Christian Kelstrup  

LAB HEAD: Jesper V. Olsen

PROVIDER: PXD006932 | Pride | 2017-11-30

REPOSITORIES: Pride

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20161213_HF_DBJ_SA_Exp6B_Hela_100ng_30min_15000_01.raw Raw
20161213_HF_DBJ_SA_Exp6B_Hela_100ng_30min_15000_02.raw Raw
20161213_HF_DBJ_SA_Exp6B_Hela_100ng_30min_15000_03.raw Raw
20161213_HF_DBJ_SA_Exp6B_Hela_100ng_30min_30000_01.raw Raw
20161213_HF_DBJ_SA_Exp6B_Hela_100ng_30min_30000_02.raw Raw
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Publications

Performance Evaluation of the Q Exactive HF-X for Shotgun Proteomics.

Kelstrup Christian D CD   Bekker-Jensen Dorte B DB   Arrey Tabiwang N TN   Hogrebe Alexander A   Harder Alexander A   Olsen Jesper V JV  

Journal of proteome research 20171220 1

Progress in proteomics is mainly driven by advances in mass spectrometric (MS) technologies. Here we benchmarked the performance of the latest MS instrument in the benchtop Orbitrap series, the Q Exactive HF-X, against its predecessor for proteomics applications. A new peak-picking algorithm, a brighter ion source, and optimized ion transfers enable productive MS/MS acquisition above 40 Hz at 7500 resolution. The hardware and software improvements collectively resulted in improved peptide and pr  ...[more]