Soil metaproteomics using DNA RNA protein co-extraction method
Ontology highlight
ABSTRACT:
INSTRUMENT(S): QSTAR
ORGANISM(S): Environmental Samples
SUBMITTER: 
Camilla Thorn
LAB HEAD: Florence Abram
PROVIDER: PXD007077 | Pride | 2019-07-15
REPOSITORIES: Pride
Dataset's files
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| Camilla1athird.wiff | Wiff | |||
| Camilla1bthird.wiff | Wiff | |||
| Camilla2athird.wiff | Wiff | |||
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| Camilla3athird.wiff | Wiff |
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Publications
A robust, cost-effective method for DNA, RNA and protein co-extraction from soil, other complex microbiomes and pure cultures.
Thorn Camilla E CE Bergesch Christian C Joyce Aoife A Sambrano Gustavo G McDonnell Kevin K Brennan Fiona F Heyer Robert R Benndorf Dirk D Abram Florence F
Molecular ecology resources 20190301 2
The soil microbiome is inherently complex with high biological diversity, and spatial heterogeneity typically occurring on the submillimetre scale. To study the microbial ecology of soils, and other microbiomes, biomolecules, that is, nucleic acids and proteins, must be efficiently and reliably co-recovered from the same biological samples. Commercial kits are currently available for the co-extraction of DNA, RNA and proteins but none has been developed for soil samples. We present a new protoco ...[more]
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