Proteomics,Multiomics

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ZBTB2 as a novel reader for unmethylated DNA

ABSTRACT: In this study, we investigated the dynamics during differentiation of the in vivo binding sites of ZBTB2, a putative reader for unmethylated DNA. We performed DNA pull-downs followed by mass spectrometry, using a genomic sequence containing either unmethylated or methylated CpGs, to study the influence of DNA methylation on ZBTB2 binding. Additionally, we performed interaction proteomics to identify ZBTB2 interaction partners. We found that ZBTB2 recruits a zinc finger module of three proteins to unmethylated DNA.

OTHER RELATED OMICS DATASETS IN: GSE101802PRJNA395559PRJNA395560PRJNA395548

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Ino Karemaker  

LAB HEAD: Michiel Vermeulen

PROVIDER: PXD007099 | Pride | 2018-01-29

REPOSITORIES: Pride

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Dataset's files

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20160311_Q1_QC_InKa_GFPpulldown_Zbtb2_BAC_IB10_GFP_1.raw Raw
20160311_Q1_QC_InKa_GFPpulldown_Zbtb2_BAC_IB10_GFP_2.raw Raw
20160311_Q1_QC_InKa_GFPpulldown_Zbtb2_BAC_IB10_GFP_3.raw Raw
20160311_Q1_QC_InKa_GFPpulldown_Zbtb2_BAC_IB10_nonGFP_1.raw Raw
20160311_Q1_QC_InKa_GFPpulldown_Zbtb2_BAC_IB10_nonGFP_2.raw Raw
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Publications

ZBTB2 reads unmethylated CpG island promoters and regulates embryonic stem cell differentiation.

Karemaker Ino D ID   Vermeulen Michiel M  

EMBO reports 20180201 4

Proteins that bind to DNA depending on its methylation status play an important role in methylation-mediated regulation of gene expression. Using a variety of genomics and proteomics approaches, we identify zinc finger and BTB domain-containing protein 2 (ZBTB2) as a reader of unmethylated DNA in mouse embryonic stem cells. ZBTB2 preferentially binds to CpG island promoters, where it acts as a transcriptional activator. The binding of ZBTB2 to its targets is direct and independent of two other z  ...[more]

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