Dataset Information

Identification of new protein-coding genes with a potential role in the virulence of the plant pathogen Xanthomonas campestris pv. vesicatoria

ABSTRACT: Bacteria of the genus Xanthomonas are economically important plant pathogens. Pathogenicity of Xanthomonas spp. depends on the type III-secretion system and additional virulence determinants. The number of sequenced Xanthomonas genomes increases rapidly, however, accurate annotation of these genomes is difficult, because it relies on gene prediction programs. In this study, we used a mass-spectrometry (MS)-based approach to identify the proteome of Xanthomonas campestris pv. vesicatoria (Xcv) also known as X. euvesicatoria, a well-studied member of plant-pathogenic Xanthomonadaceae. Results Using different culture conditions, MS-datasets were searched against a six-frame-translated genome database of Xcv. In total, we identified 2,593 proteins covering 55% of the Xcv genome, including 764 hitherto hypothetical proteins. Our proteogenomic approach identified 30 new protein-coding genes and allowed correction of the N-termini of 50 protein-coding genes. For five novel and two N-terminally corrected genes the corresponding proteins were confirmed by immunoblot. Furthermore, our data indicate that two putative type VI-secretion systems encoded in Xcv play no role in bacterial virulence which was experimentally confirmed.

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Elite

ORGANISM(S): Xanthomonas Campestris Pv. Vesicatoria Str. 85-10

SUBMITTER: Andreas Otto

LAB HEAD: Andreas Otto

PROVIDER: PXD007140 | Pride | 2017-08-17

REPOSITORIES: pride

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			Action	DRS
	130302_O1_U3_MM_UA_WT1_Ngg_01.RAW	Raw
	130302_O1_U3_MM_UA_WT1_Ngg_02.RAW	Raw
	130302_O1_U3_MM_UA_WT1_Ngg_03.RAW	Raw
	130302_O1_U3_MM_UA_WT1_Ngg_04.RAW	Raw
	130302_O1_U3_MM_UA_WT1_Ngg_05.RAW	Raw

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Identification of new protein-coding genes with a potential role in the virulence of the plant pathogen Xanthomonas euvesicatoria.

Abendroth Ulrike U Adlung Norman N Otto Andreas A Grüneisen Benjamin B Becher Dörte D Bonas Ulla U

BMC genomics 20170816 1

<h4>Background</h4>Bacteria of the genus Xanthomonas are economically important plant pathogens. Pathogenicity of Xanthomonas spp. depends on the type III-secretion system and additional virulence determinants. The number of sequenced Xanthomonas genomes increases rapidly, however, accurate annotation of these genomes is difficult, because it relies on gene prediction programs. In this study, we used a mass-spectrometry (MS)-based approach to identify the proteome of Xanthomonas euvesicatoria (X ...[more]

PMID: 28814272

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