Dataset Information

The proteome of germlings from urediniospores of Puccinia triticina

ABSTRACT: Wheat leaf rust is a serious fungal disease of wheat that causes annual losses and necessitates using fungicides for effective disease management. It is caused by Puccinia triticina which spreads by means of airborne urediniospores. When these germinate on the leaf surface, they form germ-tubes which enter the leaf through open stomates. Spores and germ-tubes represent the first fungal structures that the host can perceive during a rust infection. They therefore contain proteins that could potentially trigger early host defense responses. Using 2-DE to separate this proteome, we produced gels containing 173 spots in the pI range of 4-7 and identified 123 proteins. These were predominantly proteins involved in metabolic and cellular processes, but with a large number (77%) of novel proteins that could not be identified through homology matching Twenty four of these showed no homology to wheat sequences, making them good candidate PAMPs.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Puccinia Triticina 1-1 Bbbd Race 1

TISSUE(S): Fungal Asexual Spore

SUBMITTER: Christof Rampitsch

LAB HEAD: Christof Rampitsch

PROVIDER: PXD007162 | Pride | 2022-02-28

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Asli_Rust_spot100.mgf	Mgf
	Asli_Rust_spot100.raw	Raw
	Asli_Rust_spot101_151117112417.mgf	Mgf
	Asli_Rust_spot101_151117112417.raw	Raw
	Asli_Rust_spot104_151117130743.mgf	Mgf

Items per page:

1 - 5 of 507

Similar Datasets

Project description:Lysosomes represent a central degradative compartment of eukaryotes, yet little is known about biogenesis and function of this organelle in parasitic protists. Whereas the mannose-6 phosphate (M6P)-dependent system is dominant for lysosomal targeting in metazoans, oligosaccharide independent sorting was reported in other eukaryotes. In this study, we investigated the phagolysosomal proteome of the human parasite Trichomonas vaginalis, protein targeting and involvement of lysosomes in hydrolase secretion. The organelles were purified using conventional Percoll and OptiPrep density gradient centrifugation, and a novel purification protocol based on phagocytosis of lactoferrin-covered magnetic nanoparticles. The analysis resulted in the lysosomal proteome of 462 proteins, which were sorted into 21 functional classes. Hydrolases represent the largest functional class and include proteases, lipases, phosphatases, glycosidases, and other hydrolases. Identification of a large set of proteins involved in vesicular trafficking (80) and turnover of actin cytoskeleton rearrangement (29) indicate high dynamics of phagolysosomal compartment. Several lysosomal proteins including the cysteine protease TvCP2 were previously shown to be secreted. Our experiment showed that secretion of TvCP2 was strongly inhibited by chloroquine that increased intralysosomal pH and thus indicated TvCP2 secretion through lysosomes rather than the classical secretory pathway. Unexpectedly, we identified in the phagolysosomal proteome divergent homologs of M6P receptor TvMPR, although T. vaginalis lacks enzymes for M6P formation. To test whether oligosaccharides are involved in lysosomal targeting, we selected the lysosome-resident cysteine protease CLCP that possesses two glycosylation sites. Mutation of any of the sites redirected CLCP to the secretory pathway. Similarly, introduction of glycosylation sites to secreted β-amylase redirected this protein to lysosomes. Thus, unlike other parasitic protists, T. vaginalis seems to utilize glycosylation as recognition marker for lysosomal hydrolases. Whether TvMPR or other possible receptors are involved in this process and what is the precise structure of the lysosomal recognition marker need to be clarified in future studies.

Dataset Information

The proteome of germlings from urediniospores of Puccinia triticina

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets