Dataset Information

QUANTITATIVE PHOSPHOPROTEOMIC PROFILING OF PRIMARY RETINOBLASTOMA TUMORS

ABSTRACT: Retinoblastoma is a malignant tumor of the retina which most often occurs in children below 5 years of age with an incident rate of about 1 in 15,000 to 18,000 live births. Retinoblastoma is the first ever cancer that was reported to have a genetic basis. It occurs widely due to inactivating mutations in RB1 gene. Gene expression studies, copy number variation analysis, epigenetic profiling including miRNA and methylation of retinoblastoma has been carried to understand the disease mechanism and key players in the disease. Our group has earlier performed differential proteomics of retinoblastoma to identify proteins of therapeutic importance. However, there are no studies to understand the signalling mechanisms associated with retinoblastoma. Hence, global phosphoproteomics of retinoblastoma was carried out to identify signalling events associated with this cancer. Our study identified stress response proteins to be hyper phosphorylated which included H2AFX and sirtuin 1. In particular, Ser140 of H2AFX also known as gamma-H2AX was found to be hyperphosphorylated in retinoblastoma that indicated activation of DNA damage response pathways. We also observed activation of anti-apoptotic proteins in retinoblastoma compared to control. These observations showed activation of survival pathways and signalling networks activated in tumors.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Retina

DISEASE(S): Retinoblastoma

SUBMITTER: Sailaja Elchuri

LAB HEAD: Sailaja Elchuri

PROVIDER: PXD007228 | Pride | 2019-11-11

REPOSITORIES: Pride

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Publications

Phosphoproteomics of Retinoblastoma: A Pilot Study Identifies Aberrant Kinases.

Selvan Lakshmi Dhevi Nagarajha LDN Danda Ravikanth R Madugundu Anil K AK Puttamallesh Vinuth N VN Sathe Gajanan J GJ Krishnan Uma Maheswari UM Khetan Vikas V Rishi Pukhraj P Prasad Thottethodi Subrahmanya Keshava TSK Pandey Akhilesh A Krishnakumar Subramanian S Gowda Harsha H Elchuri Sailaja V SV

Molecules (Basel, Switzerland) 20180615 6

Retinoblastoma is a malignant tumour of the retina which most often occurs in children. Earlier studies on retinoblastoma have concentrated on the identification of key players in the disease and have not provided information on activated/inhibited signalling pathways. The dysregulation of protein phosphorylation in cancer provides clues about the affected signalling cascades in cancer. Phosphoproteomics is an ideal tool for the study of phosphorylation changes in proteins. Hence, global phospho ...[more]

PMID: 29914080

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			Action	DRS
	PRB_Phospho_1.msf	Msf
	PRB_Phospho_1.raw	Raw
	PRB_Phospho_10.msf	Msf
	PRB_Phospho_10.raw	Raw
	PRB_Phospho_11.msf	Msf