Dataset Information

Pseudomonas aeruginosa BONCAT biofilms

ABSTRACT: Cell-selective BONCAT analysis of proteins synthesized by a P. aeruginosa biofilm subpopulation throughout treatment with ciprofloxacin.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Pseudomonas Aeruginosa (strain Ucbpp-pa14)

SUBMITTER: Brett Babin

LAB HEAD: David A. Tirrell

PROVIDER: PXD007261 | Pride | 2017-10-24

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	MaxQuant_output.zip	Other
	NLL-mCherry.fasta	Fasta
	PA14_151116.fasta	Fasta
	brb_rpoS_cipro00_enrA_16November15.raw	Raw
	brb_rpoS_cipro00_enrB_16November15.raw	Raw

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Publications

Selective Proteomic Analysis of Antibiotic-Tolerant Cellular Subpopulations in <i>Pseudomonas aeruginosa</i> Biofilms.

Babin Brett M BM Atangcho Lydia L van Eldijk Mark B MB Sweredoski Michael J MJ Moradian Annie A Hess Sonja S Tolker-Nielsen Tim T Newman Dianne K DK Tirrell David A DA

mBio 20171024 5

Biofilm infections exhibit high tolerance against antibiotic treatment. The study of biofilms is complicated by phenotypic heterogeneity; biofilm subpopulations differ in their metabolic activities and their responses to antibiotics. Here, we describe the use of the bio-orthogonal noncanonical amino acid tagging (BONCAT) method to enable selective proteomic analysis of a <i>Pseudomonas aeruginosa</i> biofilm subpopulation. Through controlled expression of a mutant methionyl-tRNA synthetase, we t ...[more]

PMID: 29066549

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