Dataset Information

Proteomics analysis of milk from cows with Staphylococcus aureus subclinical mastitis

ABSTRACT: Liquid chromatography-mass spectrometry (LC/MS) based label free quantitative proteomics analysis was applied for identification of differentially expressed proteins among the whey samples isolated from, 1) milk from cows with no history of mastitis, negative for S. aureus and somatic cell count (SCC) >2×105 cells\ml and 2) milk samples from sub-clinical mastitis., i.e., positive for S. aureus and SCC >2×105 cells\ml.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Bos Taurus (bovine) Bos Taurus

TISSUE(S): Milk

DISEASE(S): Mastitis

SUBMITTER: Jayaseelan Murugaiyan

LAB HEAD: Jayaseelan Murugaiyan

PROVIDER: PXD007516 | Pride | 2018-01-02

REPOSITORIES: Pride

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Publications

Identification of Host Defense-Related Proteins Using Label-Free Quantitative Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus Subclinical Mastitis.

Abdelmegid Shaimaa S Murugaiyan Jayaseelan J Abo-Ismail Mohamed M Caswell Jeff L JL Kelton David D Kirby Gordon M GM

International journal of molecular sciences 20171228 1

<i>Staphylococcus aureus</i> is the most common contagious pathogen associated with bovine subclinical mastitis. Current diagnosis of <i>S. aureus</i> mastitis is based on bacteriological culture of milk samples and somatic cell counts, which lack either sensitivity or specificity. Identification of milk proteins that contribute to host defense and their variable responses to pathogenic stimuli would enable the characterization of putative biomarkers of subclinical mastitis. To accomplish this, ...[more]

PMID: 29283389

Similar Datasets

Project description:Bovine mastitis, the infection of the mammary gland which leads to great health and economic challenges for dairy farmers is accompanied by dramatic changes in the milk proteome. In this study of naturally occurring mastitis not only have the changes in the milk proteome been quantified in subclinical and clinical mastitis but simultaneous changes in the serum proteome have also been characterised and quantified. Milk and serum samples from healthy dairy cows (n=12) were compared to those of cows with subclinical (n=10) and clinical mastitis (n=112) using TMT label-based proteomic approach. The study included the milk and serum samples taken from thirty-two dairy cows ( kept on private farms located in Croatia. All cows were checked by physical examination. Somatic cells count (SCC) and mastitis test in milk samples were performed. According to the results, cows were assigned into three groups: Group I (control, n=10) consisted of healthy cows with SCC below 400,000 cells/ml on the monthly check-up and a negative mastitis test and without any clinical sign of mastitis. Group II (subclinical mastitis, n=12) comprised cows without clinical signs of mastitis but with SCC above 400,000 cells/ml on the monthly basis and a positive mastitis test at the time of sampling. Group III (clinical mastitis, n=10) consisted of cows with clinical signs of mastitis which include changes in milk appearance (flakes and clots in milk), different stages of udder inflammation (hyperemia, edema, pain, udder enlargement and elevated udder temperature) and disturbance of general health (depression, relaxed cold ears, dehydration, elevated body temperature, increased heart and respiratory rate, decreased ruminal contraction and decreased appetite). Blood samples were taken from v. coccygea and centrifuged at 3000 g for 15 min after clotting for two hours at room temperature. Serum samples were stored at -80°C until analysis. Milk samples were taken aseptically before the morning milking. First few streams were discarded. Teat ends were disinfected with cotton swabs soaked with 70% ethanol. Samples were taken into sterile tubes and transported to laboratory on ice within a few hours.

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