Dataset Information

Arabidopsis diurnal LFQ proteome and labelled phosphoproteome

ABSTRACT: Plant growth and survival depends to a large extent on the diurnal regulation of cellular processes. Although extensively studied at the transcript level, notably less is known about diurnal fluctuations at the protein level. Here, we report a high-resolution quantitative time-course of the Arabidopsis rosette proteome and phosphoproteome over a 12 h light:12 h dark diel cycle. We monitored the proteome every 2 h and the phosphoproteome immediately before and after the light-to-dark and dark-to-light transitions. Notably, we quantified nearly 5000 proteins and 1800 phosphopeptides, of which 288 and 225, respectively, were found to fluctuate over the time-course. Diurnal proteome and phosphoproteome changes were related to diverse biological processes, including protein translation, light detection, photosynthesis, metabolism and transport. Together, these datasets represent the most comprehensive proteomic analysis of Arabidopsis rosettes to date, allowing us to make multi-level inferences about the diurnal regulation of key cellular plant processes plants.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Rosette

SUBMITTER: Sira Echevarria-Zomeno

LAB HEAD: Prof. Wilhelm Gruissem

PROVIDER: PXD007600 | Pride | 2020-12-18

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20141209_02_27_30min_BL_1_P.raw	Raw
	20141209_03_19_10min_AD_2_P.raw	Raw
	20141209_05_03_10min_AD_3_P.raw	Raw
	20141209_06_12_30min_AD_3_P.raw	Raw
	20141209_07_21_10min_AL_4_P.raw	Raw

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Publications

Diurnal dynamics of the Arabidopsis rosette proteome and phosphoproteome.

Uhrig R Glen RG Echevarría-Zomeño Sira S Schlapfer Pascal P Grossmann Jonas J Roschitzki Bernd B Koerber Niklas N Fiorani Fabio F Gruissem Wilhelm W

Plant, cell & environment 20201224 3

Plant growth depends on the diurnal regulation of cellular processes, but it is not well understood if and how transcriptional regulation controls diurnal fluctuations at the protein level. Here, we report a high-resolution Arabidopsis thaliana (Arabidopsis) leaf rosette proteome acquired over a 12 hr light:12 hr dark diurnal cycle and the phosphoproteome immediately before and after the light-to-dark and dark-to-light transitions. We quantified nearly 5,000 proteins and 800 phosphoproteins, of ...[more]

PMID: 33278033

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