Proteomics

Dataset Information

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Maize and Arabidopsis Golgi proteome

ABSTRACT: Comparative proteomics of ER-Golgi membranes from etiolated coleoptiles of maize (Zea mays) and young leaves of Arabidopsis (Arabidopsis thaliana) isolated by flotation centrifugation demonstrate differences in the complement of synthases and glycosyl transferases unique to the type of wall made. Additionally, maize Golgi membranes enriched by flotation were separated further by free-flow electrophoresis (FFE), yielding 3009 proteins, of which 229 were known to function in cell wall synthesis or metabolism. A subset of proteins identified after flotation centrifugation were identified in Golgi membranes but failed to enrich in them. Individual classes of proteins associated with cell wall synthesis were asymmetrically distributed across the fractions of Golgi membranes separated by FFE.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) Zea Mays (maize)

TISSUE(S): Plant Cell, Etiolated Plant Tissue, Leaf

DISEASE(S): Disease Free

SUBMITTER: Ikenna Okekeogbu  

LAB HEAD: Nicholas Carpita

PROVIDER: PXD007612 | Pride | 2019-04-11

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
072815_Ikenna_Golgi_1_A-006.mzML Mzml
072815_Ikenna_Golgi_1_A.mgf Mgf
072815_Ikenna_Golgi_1_A.pride.mgf.gz Mgf
080516_ikenna_Arab-golgi_1_A-013.mzML Mzml
080516_ikenna_Arab-golgi_1_A.mgf Mgf
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Publications

Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures.

Okekeogbu Ikenna O IO   Pattathil Sivakumar S   González Fernández-Niño Susana M SM   Aryal Uma K UK   Penning Bryan W BW   Lao Jeemeng J   Heazlewood Joshua L JL   Hahn Michael G MG   McCann Maureen C MC   Carpita Nicholas C NC  

The Plant cell 20190326 5

The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking of all noncellulosic polysaccharides, proteoglycans, and proteins destined for the cell wall. As grass species make cell walls distinct from those of dicots and noncommelinid monocots, it has been assumed that the differences in cell-wall composition stem from differences in biosynthetic capacities of their respective Golgi. However, immunosorbence-based screens and carbohydrate linkage analysis o  ...[more]

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