Dataset Information

Human aqueous humor of Primary open angle glaucoma LC-MS/MS

ABSTRACT: The main objective of this study was to adopt discovery based approach and define quantitative expression of human AH samples of glaucoma (N= 5) and non-glaucoma subjects (N=5) by using a state-of-the-art high performance liquid chromatography coupled mass spectrometry, to establish the relative regulation of AH proteome in glaucomatous eye and affected cellular pathways, and their correlation with disease pathogenesis

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Aqueous Drainage System, Anterior Chamber Of The Eye

SUBMITTER: Sunil Adav

LAB HEAD: Prof. Sze S K

PROVIDER: PXD007624 | Pride | 2018-06-15

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	160423_JWei_Sun_TTSH_AH_CG070_A1.raw	Raw
	160423_JWei_Sun_TTSH_AH_CG070_A2.raw	Raw
	160423_JWei_Sun_TTSH_AH_CG070_A3.raw	Raw
	160423_JWei_Sun_TTSH_AH_CG070_A4.raw	Raw
	160423_JWei_Sun_TTSH_AH_CG070_B1.raw	Raw

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Publications

Proteomic Analysis of Aqueous Humor from Primary Open Angle Glaucoma Patients on Drug Treatment Revealed Altered Complement Activation Cascade.

Adav Sunil S SS Wei Jin J Terence Yap Y Ang Bryan C H BCH Yip Leonard W L LWL Sze Siu Kwan SK

Journal of proteome research 20180626 7

Primary open angle glaucoma (POAG) is a complex disease and a leading cause of irreversible blindness, and its underlying pathophysiology remains poorly understood. Proteomic characterization of the protein composition of aqueous humor (AH) may identify prognostic candidate proteins involved in pathogenesis and progression of the disease. To delineate the possible mechanisms that lead to POAG, this study adopted state-of-art mass spectrometric technique and analyzed AH of POAG and their respecti ...[more]

PMID: 29901396

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Project description:Purpose: The optic nerve head (ONH) is the likely site of initial damage in the glaucomatous eye. Despite the recognition of elevated intraocular pressure (IOP) as a leading risk factor for the development of glaucoma, ocular hypertension (OHT) eyes displaying consistently elevated IOP do not experience ONH damage. This study aims to identify global gene expression variations in glaucomatous ONHs and their relationship to those identified in OHT derived ONHs in order to improve our understanding of IOP-induced ONH damage. Methods: (N=6) ONHs were collected from clinically confirmed glaucoma, OHT and age-matched control donor eyes. Total RNA extracted from ONHs was reverse transcribed and assayed using the Affymetrix Human Exon 1.0 ST array. Differentially expressed genes in glaucoma versus control and OHT derived ONHs were identified using an ANOVA analysis with a 1.25 fold change limit and P-value < 0.05. Quantitative RT-PCR was performed to validate selected differentially expressed genes. Results: Microarray analysis revealed 149 under under-expressed genes in POAG versus control ONHs, many of which are involved in ion transport, axonogenesis and macromolecule catabolic processes. 297 genes were over expressed in OHT versus glaucoma derived ONHs. Mediators of oxidation-reduction and chemical homeostasis were among the most prominent gene groups identified. The over expression of prostaglandin-endoperoxide synthase 2, integrin, beta-like 1 and fibulin 5 in glaucomatous ONHs was confirmed by qRT-PCR. Conclusions: Our data demonstrates marked alteration in global gene expression patterns in the glaucomatous ONH, likely due to extensive tissue injury. The observed overlapping of several differentially expressed genes in glaucoma and OHT derived ONHs suggests the induction of common mechanisms in response to elevated IOP. Preferential over-expression of certain gene groups in OHT but not glaucoma derived ONHs may confer possible protection against IOP-induced ONH damage, which remains to be investigated in future studies. (N=6) Glaucoma, ocular hypertension and age-matched control ONHs were assayed to investigate and compare global gene expression patterns in each sample group.