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UPS-2 - Increased Sensitivity of Mass Spectrometry by Alkaline Two-Dimensional Liquid Chromatography: Deep Cover of the Human Proteome in Gene-Centric Mode

ABSTRACT: using UPS-2 set as an example we characterized mass spectrometric approaches from the point of view of sensitivity (Sn), specificity (Sp) and accuracy (Ac)

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

TISSUE(S): Blood Plasma

SUBMITTER: Ekaterina Ilgisonis

LAB HEAD: Victor Zgoda

PROVIDER: PXD007643 | Pride | 2018-11-23

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	E_coli_MTRX.mgf	Mgf
	E_coli_matrix_R01.mzML	Mzml
	E_coli_matrix_R01.raw	Raw
	E_coli_matrix_R02.mzML	Mzml
	E_coli_matrix_R02.raw	Raw

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Publications

Increased Sensitivity of Mass Spectrometry by Alkaline Two-Dimensional Liquid Chromatography: Deep Cover of the Human Proteome in Gene-Centric Mode.

Ilgisonis Ekaterina V EV Kopylov Arthur T AT Ponomarenko Elena A EA Poverennaya Ekaterina V EV Tikhonova Olga V OV Farafonova Tatiana E TE Novikova Svetlana S Lisitsa Andrey V AV Zgoda Victor G VG Archakov Alexander I AI

Journal of proteome research 20181119 12

Currently, great interest is paid to the identification of "missing" proteins that have not been detected in any biological material at the protein level (PE1). In this paper, using the Universal Proteomic Standard sets 1 and 2 (UPS1 and UPS2, respectively) as an example, we characterized mass spectrometric approaches from the point of view of sensitivity (Sn), specificity (Sp), and accuracy (Ac). The aim of the paper was to show the utility of a mass spectra approach for protein detection. This ...[more]

PMID: 30354151

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