Dataset Information

Lipid droplet-targeted APEX proteome

ABSTRACT: Lipid droplet (LD) function is regulated by a complement of integral and peripheral proteins that associate with the LD phospholipid monolayer. Defining the composition of the LD proteome has remained a challenge due to the presence of contaminating proteins in LD-enriched buoyant fractions. To overcome this limitation, we developed a proximity labeling strategy that exploits LD-targeted APEX2 to biotinylate LD proteins in living cells. Application of this approach to U2OS and Huh7 cells identified the vast majority of previously validated LD proteins, excluded common contaminating proteins, and revealed new LD proteins.

INSTRUMENT(S): 6410 Triple Quadrupole LC/MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: James Olzmann

LAB HEAD: James Olzmann

PROVIDER: PXD007695 | Pride | 2017-12-22

REPOSITORIES: Pride

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Publications

A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes.

Bersuker Kirill K Peterson Clark W H CWH To Milton M Sahl Steffen J SJ Savikhin Victoria V Grossman Elizabeth A EA Nomura Daniel K DK Olzmann James A JA

Developmental cell 20171221 1

Lipid droplet (LD) functions are regulated by a complement of integral and peripheral proteins that associate with the bounding LD phospholipid monolayer. Defining the composition of the LD proteome has remained a challenge due to the presence of contaminating proteins in LD-enriched buoyant fractions. To overcome this limitation, we developed a proximity labeling strategy that exploits LD-targeted APEX2 to biotinylate LD proteins in living cells. Application of this approach to two different ce ...[more]

PMID: 29275994

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			Action	DRS
	ATGL-Apex2-Huh7-I.raw	Raw
	ATGL-Apex2-Huh7-II.raw	Raw
	ATGL-Apex2-U2OS-I.raw	Raw
	ATGL-Apex2-U2OS-II.raw	Raw
	Cyto-Apex2-Huh7-I.raw	Raw