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DYRK3 protein-protein interactions in human cells

ABSTRACT: Quantitative affinity purification experiment was performed to identify the protein interactors of DYRK3 kinase in human cells. An additional pull-down experiment was carried out to study the changes of DYRK3 interactors with GSK-626616 kinase inhibitor treatment.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Jia-Xuan Chen

LAB HEAD: Matthias Selbach

PROVIDER: PXD007761 | Pride | 2018-07-05

REPOSITORIES: Pride

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			Action	DRS
	MaxQuant_Output_DYRK3_PPI.zip	Other
	MaxQuant_Output_DYRK3_PPI_kinase_inhibitor.zip	Other
	Statler_20141212_JC_IP20141210_EGFPDYRK3_FOR.raw	Raw
	Statler_20141212_JC_IP20141210_EGFPDYRK3_REV.raw	Raw
	Waldorf_20150607_JC_Arpan_IP20150603_DYRK3_GSK.raw	Raw

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Publications

Kinase-controlled phase transition of membraneless organelles in mitosis.

Rai Arpan Kumar AK Chen Jia-Xuan JX Selbach Matthias M Pelkmans Lucas L

Nature 20180704 7713

Liquid-liquid phase separation has been shown to underlie the formation and disassembly of membraneless organelles in cells, but the cellular mechanisms that control this phenomenon are poorly understood. A prominent example of regulated and reversible segregation of liquid phases may occur during mitosis, when membraneless organelles disappear upon nuclear-envelope breakdown and reappear as mitosis is completed. Here we show that the dual-specificity kinase DYRK3 acts as a central dissolvase of ...[more]

PMID: 29973724

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