Dataset Information

Effect of Sec61 inhibitor mycolactone on the proteome of the MED17.11 neuronal cell line

ABSTRACT: Mycolactone is a mycobacteria-derived macrolide that blocks the biogenesis of a large array of secreted and transmembrane proteins through potent inhibition of the Sec61 translocon. Here, we used quantitative proteomics to delineate the direct and indirect effects of mycolactone-mediated Sec61 blockade on mouse on MED17.11 cells, as a model of dorsal root ganglia (DRG) sensory neurons, in resting and LPS-stimulated conditions. This analysis completes two previously reported ones, which investigated the effects of mycolactone on the proteome of the mouse MutuDC dendritic cells (Project PXD006103) and human Jurkat T cells (Project PXD002971).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Permanent Cell Line Cell, Cell Culture, Sensory Neuron Of Dorsal Root Ganglion

SUBMITTER: Jean-David Morel

LAB HEAD: Caroline Demangel

PROVIDER: PXD007770 | Pride | 2018-06-26

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Maxquantoutputfiles.zip	Other
	Perseusworflowandoutputfiles.zip	Other
	Rawfiles.zip	Other
	Rscriptlimmaanalysisandinputandoutputfiles.zip	Other
	Summaryofresults.xlsx	Xlsx

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Publications

Proteomics Reveals Scope of Mycolactone-mediated Sec61 Blockade and Distinctive Stress Signature.

Morel Jean-David JD Paatero Anja O AO Wei Jiajie J Yewdell Jonathan W JW Guenin-Macé Laure L Van Haver Delphi D Impens Francis F Pietrosemoli Natalia N Paavilainen Ville O VO Demangel Caroline C

Molecular & cellular proteomics : MCP 20180618 9

Mycolactone is a bacteria-derived macrolide that blocks the biogenesis of a large array of secretory and integral transmembrane proteins (TMP) through potent inhibition of the Sec61 translocon. Here, we used quantitative proteomics to delineate the direct and indirect effects of mycolactone-mediated Sec61 blockade in living cells. In T lymphocytes, dendritic cells and sensory neurons, Sec61 substrates downregulated by mycolactone were in order of incidence: secretory proteins (with a signal pept ...[more]

PMID: 29915147

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