Dataset Information

Immunoproteomic analysis of the excretory-secretory products of the Trichinella pseudospiralis adult worms and newborn larvae

ABSTRACT: In this study, we used Two-dimensional gel electrophoresis (2-DE)-based Western blotting coupled with MALDI-TOF / TOF-MS / MS to separate and identify the T. pseudospiralis adult worms (Ad) ES products immunoreaction-positive proteins. In total, 400 protein spots were separated by 2-DE. 28 protein spots were successfully identified using the sera from infected pigs and were characterized to correlate with twelve different proteins of T. pseudospiralis.

INSTRUMENT(S): ultraflex

ORGANISM(S): Trichinella Pseudospiralis

SUBMITTER: yang wang

LAB HEAD: ming yuan liu

PROVIDER: PXD007783 | Pride | 2018-11-22

REPOSITORIES: Pride

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Immunoproteomic analysis of the excretory-secretory products of Trichinella pseudospiralis adult worms and newborn larvae.

Wang Yang Y Bai Xue X Zhu Haichao H Wang Xuelin X Shi Haining H Tang Bin B Boireau Pascal P Cai Xuepeng X Luo Xuenong X Liu Mingyuan M Liu Xiaolei X

Parasites & vectors 20171121 1

<h4>Background</h4>The nematode Trichinella pseudospiralis is an intracellular parasite of mammalian skeletal muscle cells and exists in a non-encapsulated form. Previous studies demonstrated that T. pseudospiralis could induce a lower host inflammatory response. Excretory-secretory (ES) proteins as the most important products of host-parasite interaction may play the main functional role in alleviating host inflammation. However, the ES products of T. pseudospiralis early stage are still unknow ...[more]

PMID: 29157262

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