Proteomics

Dataset Information

0

Apis mellifera,Hemolymph,LC-MSMS


ABSTRACT: We characterized and compared hemolymph proteome of Royal Jelly bees (RJbs), a stock selected for increasing RJ output from Italian bees (ITbs) and ITbs across the larval and adult ages. Unprecedented depth of proteome was attained by identifying 3394 hemolymph proteins in both bee lines. The proteome supports the general function of hemolymph to drive development and immunity across different phases in both bees. However, age-specific proteome settings have adapted to prime the distinct physiology for larvae and adult bees. In larvae, proteome are thought to drive the temporal immunity, rapid organogenesis, and reorganization of larval structures. In adults, proteome play key roles to prompt tissues development and immune defense in NEBs, glands maturity in NBs and carbohydrate energy production in FBs. Comparing the proteome between the same aged larval and adult samples, RJbs and ITbs have tailored distinct hemolymph proteome programs to drive their physiology. Particularly, in day 4 larvae and NBs, a large number of highly abundant proteins enriched in protein synthesis and energy metabolism in RJbs relative to ITbs imply that RJb larvae and NBs have reprogrammed their proteome to initiate different developmental trajectory and high RJ secretion in response to the enhanced RJ production by selection. Our hitherto depth of proteome coverage gains novel sight on molecular details in driving hemolymph function and high RJ production by RJbs.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Apis Mellifera (honeybee)

TISSUE(S): Blood

SUBMITTER: Zewdu hora  

LAB HEAD: Jianke Li

PROVIDER: PXD007824 | Pride | 2017-11-30

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
ITB2d2.mzxml Mzxml
ITB2d2.pep.xml Pepxml
ITB2d2.raw Raw
ITB2d3.mzxml Mzxml
ITB2d3.pep.xml Pepxml
Items per page:
1 - 5 of 108
altmetric image

Publications

Proteome Comparisons between Hemolymph of Two Honeybee Strains (Apis mellifera ligustica) Reveal Divergent Molecular Basis in Driving Hemolymph Function and High Royal Jelly Secretion.

Ararso Zewdu Z   Ma Chuan C   Qi Yuping Y   Feng Mao M   Han Bin B   Hu Han H   Meng Lifeng L   Li Jianke J  

Journal of proteome research 20171205 1


Hemolymph is vital for the immunity of honeybees and offers a way to investigate their physiological status. To gain novel insight into the functionality and molecular details of the hemolymph in driving increased Royal Jelly (RJ) production, we characterized and compared hemolymph proteomes across the larval and adult ages of Italian bees (ITbs) and Royal Jelly bees (RJbs), a stock selected from ITbs for increasing RJ output. Unprecedented in-depth proteome was attained with the identification  ...[more]

Similar Datasets

2016-08-09 | PXD004467 | Pride
2014-05-20 | E-GEOD-47136 | biostudies-arrayexpress
2013-07-05 | E-MEXP-3608 | biostudies-arrayexpress
2013-09-01 | E-MTAB-1671 | biostudies-arrayexpress
2014-06-01 | E-MTAB-2621 | biostudies-arrayexpress
2019-01-17 | PXD011997 | Pride
2013-06-01 | E-MTAB-1285 | biostudies-arrayexpress
2014-11-12 | PXD001243 | Pride
2013-09-01 | E-MTAB-1675 | biostudies-arrayexpress
2016-03-31 | E-MTAB-4142 | biostudies-arrayexpress