A systematic evaluation of the use of human plasma and serum for mass spectrometry-based shotgun proteomics - commercial pooled samples

ABSTRACT: Over the last two decades, EDTA-plasma has been used as the preferred sample matrix for human blood proteomic profiling. Serum has also been employed widely. Only a few studies have assessed the difference and relevance of the proteome profiles obtained from plasma samples, such as EDTA-plasma or lithium-heparin-plasma, and serum. A complete evaluation of the use of EDTA-plasma, heparin-plasma, and serum would greatly expand the comprehensiveness of shotgun proteomics of blood samples. In this study, we evaluated the use of heparin-plasma with respect to EDTA-plasma and serum to profile blood proteomes using a scalable automated proteomic pipeline (ASAP2). The use of plasma and serum for mass spectrometry-based shotgun proteomics was tested with commercial pooled samples. The proteome coverage consistency and the quantitative performance were compared.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma, Blood Serum

SUBMITTER: Loïc Dayon  

LAB HEAD: Loïc Dayon

PROVIDER: PXD007846 | Pride | 2018-02-27

REPOSITORIES: Pride