Dataset Information

Myristic acid induces proteomic and secretomic changes associated with steatosis, cytoskeleton remodeling, endoplasmatic reticulum stress and exosome release in HepG2 cells

ABSTRACT: Myristic acid, the 14-carbon saturated fatty acid (C14:0), is usually associated with negative consequences for human health, and in particular its consumption is correlated to an increased cardiovascular disease risk. Since it is a little abundant into the cells, its specific properties and functional roles have not been fully described. The aim of this study was to explore the cell response to this fatty acid to help explain clinical findings on the relationship between C14:0 and cardiovascular disease. The human liver HepG2 cell line was used as a model to investigate the hepatic response to C14:0 in a combined proteomic and secretomic approach. A total of 47 intracellular and 32 secreted proteins were found to be deregulated among the different dosages of C14:0 treatments (50, 125, and 250 μM) as compared to control. Bioinformatics analysis revealed that myristic acid modulates the lipid droplet formation and the cytoskeleton organization, in addition it induces ER stress as well as changes in exosome and extracellular miRNA sorting. Our data provide for the first time quantitative proteomic data regarding C14:0 and contribute to the explanation of the molecular mechanisms through which this fatty acid can cause adverse health effects.

INSTRUMENT(S): Bruker Daltonics maXis series

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepg2 Cell

SUBMITTER: Barbara Darnhofer

LAB HEAD: Ruth Birner-Gruenberger

PROVIDER: PXD007902 | Pride | 2018-04-17

REPOSITORIES: Pride

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			Action	DRS
	0A_nuovo_lisato_BD1_01_3752.d.zip	Other
	0B_nuovo_lisato_BD2_01_3754.d.zip	Other
	0C_nuovo_lisato_BD3_01_3756.d.zip	Other
	125A_nuovo_lisato_BD7_01_3764.d.zip	Other
	125B_nuovo_lisato_BD8_01_3768.d.zip	Other

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Myristic acid induces proteomic and secretomic changes associated with steatosis, cytoskeleton remodeling, endoplasmic reticulum stress, protein turnover and exosome release in HepG2 cells.

Speziali Giulia G Liesinger Laura L Gindlhuber Juergen J Leopold Christina C Pucher Bettina B Brandi Jessica J Castagna Annalisa A Tomin Tamara T Krenn Petra P Thallinger Gerhard G GG Olivieri Oliviero O Martinelli Nicola N Kratky Dagmar D Schittmayer Matthias M Birner-Gruenberger Ruth R Cecconi Daniela D

Journal of proteomics 20180412

Myristic acid, the 14-carbon saturated fatty acid (C14:0), is associated to an increased cardiovascular disease risk. Since it is found in low concentration in cells, its specific properties have not been fully analyzed. The aim of this study was to explore the cell response to this fatty acid to help explaining clinical findings on the relationship between C14:0 and cardiovascular disease. The human liver HepG2 cell line was used to investigate the hepatic response to C14:0 in a combined proteo ...[more]

PMID: 29654920

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